Pak. J. Bot., 47(6): 2361-2367, 2015.
Axenic Seed Culture and in vitro mass propagation of Malaysian Wild Orchid Cymbidium finlaysonianum LINDL.
Touhidul Islam1, Bakul Bhattacharjee1, S.M. Shahinul Islam1*, Jasim Uddain2 AND Sreeramanan Subramaniam2
1Plant Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh
2School of Biological Sciences, University Sains Malaysia (USM), 11800 Gelugor, Penang, Malaysia
*Correspondence author: shahin_ibsc@ru.ac.bd
Abstract: Under this study an efficient protocol on mass propagation of Cymbidium finlaysonianum an epiphytic Malaysian wild orchid has been established using axenic culture. To obtain an axenic seed culture, it is important to perform an adequate a disinfection procedure in tissue culture. Four nutrient media viz. MS, ˝MS, KC and VW were evaluated on In vitro seed germination with callus initiation. The maximum seed germination with callus initiation (100%) was recorded in MS basal medium with a short span of time (40 days after culture). After 45 days of callus initiation the effect of eight different treatments (T1-T8) on callus size and nature were also studied. The experiment revealed that in T3 (MS + 2.0 mgl-1 BAP + 0.5 mgl-1 NAA) was found to be the best for callus development (1.98 cm length and 1.01 cm breadth). The effect of different concentration of BAP was evaluated on protocorm formation and its proliferation. Maximum number (7.75) and percentage (81.40) of PLBs was recorded in MS medium supplemented with 1.5 mgl-1 BAP. Very good PLBs development was recorded also in MS + BAP 1.0 mgl-1 + NAA 0.5 mgl-1. The highest elongation of shoot (3.80 cm) was observed in MS + 1.0 mgl-1 BAP + 0.50 mgl-1 NAA. For root induction 1.0 mgl-1 NAA has proven to the best in ˝MS medium. The In developed seedlings were finally transferred to pots by successive phases of acclimatization.
Key words: Axenic culture; Cymbidium finlaysonianum; Protocorm like bodies (PLBs); Mass propagation, Media.