PJB-2022-490
Analysis of antimicrobial potential of silver nanoparticles synthesized by leaf extract of Moringa oleifera
Iqra Mujahid, Muhammad Mansha, Peter C Thomson, Anis Ali Shah and Okasha Abrar
DOI:10.30848/PJB2025-2(26)
Abstract
In the present study silver (Ag) nanoparticles synthesized from extract taken from Moringa oleifera leaves, were characterized and examined for antimicrobial properties. Color change, UV-visible spectroscopy and Fourier transform infrared (FTIR) techniques were exercised for characterization of Ag nanoparticles. Antimicrobial potential of Ag nanoparticles was analyzed using disc diffusion method against clinically important Gram-positive bacteria (Geobacillus, Staphylococcus aureus, Bacillus subtilis), Gram negative bacteria (Klebsiella pneumonia, Psuedomonas aeruginosa, Escherichia coli) and a fungal species (Candida albicans). The antimicrobial activity was assayed by size of growth inhibition zone developed by Ag nanoparticles, and its dilutions 3:1, 1:1, in comparison to the inhibition activity exhibited by positive control (amoxicillin /fluconazole) and negative control (deionized water) against the tested strains of bacteria and fungus. The largest growth inhibition zone (17.00±0.577) was observed on staphylococcus aureus among Gram-positive bacteria, while in Gram-negative bacteria, the largest inhibition zone (17.66±1.2) was obtained on Pseudomonas aerginosa. The results revealed that there is insignificant difference among zone of inhibition developed by pure silver nanoparticles solution and its dilutions (17.00±0.577, 16.00±0.577 & 15.66±0.881). Moreover, inhibition zone developed by positive control and nanoparticle solution (18.66±1.20 & 17.00±0.577) were insignificantly different. But the zone of inhibition on Candida albicans developed by nanoparticles is significantly small as compared to positive control (17.66±0.33 & 12.66±0.33). Taken together, findings of this study revealed that Ag nanoparticles showed the most significant inhibition activity (p=0.00) against the examined strains of Gram-positive and Gram-negative bacteria but this activity is poor on C. albicans as exhibited by inhibitory zone
To Cite this article:
Mujahid, I., M. Mansha, P.C. Thomson, A.A. Shah and O. Abrar. 2025. Analysis of antimicrobial potential of silver nanoparticles synthesized by leaf extract of Moringa oleifera. Pak. J. Bot., 57(2): DOI: http://dx.doi.org/10.30848/PJB2025-2(26)
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