PJB-2023-234
Optimization of rapid protocol for extraction of phytochemical from Olea europaea L. – Oleaceae
Ramish Nida, Sumera Javad, Khajista Jabeen, Iqra Akhtar, Nimra Kousar, Anis Ali Shah and Muhammad Jan
DOI:10.30848/PJB2024-6(8)
Abstract
The purpose of this study was to optimize a rapid protocol for the extraction of phytochemicals, using a microwave-assisted extraction technique (MAE) from Olea europaea L. For this purpose, fruits, seeds, and leaves of O. europaea var. Earlik were extracted by MAE. MAE parameters including power level (300-1000 W), extraction time (1-10 min), and polar (Ethanol and Acetone) and Non-polar solvents (n-hexane) ratios (8.65 -10 mL) for maximum phenolic and flavonoid content were optimized using central composite design (CCD). Extracts with non-polar solvent (n-hexane) and esterified olive oil were analyzed by GC-MS. After that, the extracts with maximum phenolics were analyzed and compared for antioxidant and antifungal activities. Results showed that leaf extracts in 4.5 minutes, 1000 W, and 10 mL ethanol contained higher phenolics and flavonoids. Leaf extract also showed higher antioxidant potential and higher antifungal potential against Aspergillus flavus. Same biological and phytochemical assays were repeated for microwave-assisted extracts of olive seeds and fruits with promising results. Parameters that showed the optimized results were power level (600 W), solvent (10 mL) and solvent (ethanol). Overall leaf extract showed higher antifungal and antioxidant potential compared to seed and fruit extract using the optimized extraction parameters with leaves. GC-MS analysis showed 20, 21, 16, and 8 components from fruit, seed, leaf and olive oil respectively. The results of the study demonstrated that MAE was a fast and energy-efficient method for extracting both polar and non-polar components from olives and can be used as an extraction method for olives which can lead to savings in time, energy, and cost. This rapid method of extraction can be used to analyze the phytochemicals from various other varieties of olives.
To Cite this article:
Nida, R., S. Javad, K. Jabeen, I. Akhtar, N. Kousar, A.A. Shah and M. Jan. 2024. Optimization of rapid protocol for extraction of phytochemical from Olea europaea L. – Oleaceae. Pak. J. Bot., 56(6): DOI: http://dx.doi.org/10.30848/PJB2024-6(8)
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