PJB-2023-480
A cytological study on the meiosis process of Humulus scandens
Jinjin Guo, Yi Liang, Deqing Meng, Mengyi Wang, Yaning Liu, Geyang Wu, Jinhong Yuan and Junhua Li
DOI:10.30848/PJB2024-6(40)
Abstract
Humulus scandens is a common dioecious weed that possesses valuable forage and medicinal properties. It exhibits strong adaptability and a high seed yield, making it a subject of significant research interest. However, there is a lack of information concerning the meiosis process of H. scandens and the relevant methods used for studying it. In this study, we aimed to address this gap by utilizing intact H. scandens meiosis cells as research material. We employed a rapid labeling method to observe the meiotic chromosomes. Additionally, a single oligonucleotide probe of 5S rDNA was used to asertain that the number of 5S rDNA signals in the somatic cells of H. scandens was one pair. Using this probe, we conducted an inspection of homologous chromosome pairing in H. scandens. Our study provides a comprehensive description of the meiosis process in H. scandens and introduces a set of cytological methods suitable for the easy labeling of meiotic chromosomes and the monitoring of homologous chromosome pairing in H. scandens
To Cite this article:
Guo, J., Y. Liang, D. Meng, M. Wang, Y. Liu, G. Wu, J. Yuan and J. Li. 2024. A cytological study on the meiosis process of Humulus scandens. Pak. J. Bot., 56(6): DOI: http://dx.doi.org/10.30848/PJB2024-6(40)
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