PJB-2023-546
Comparison of the effects of seven hybrid combinations of pumpkin root stocks on the growth and antioxidant enzyme activity of grafted cucumber
Rui Chen, Volodymyr Dubovyk, Bihua Chen and Jinyi Liu
DOI:10.30848/PJB2025-3(37)
Abstract
At present, melon crops suffer significant losses due to long-term continuous cropping, which can be overcome in production by selecting excellent rootstocks for grafting. This study used seven different hybrid combinations of pumpkin rootstocks as materials. We measured growth physiological indicators by grafting with cucumbers, including: Measuring plant height with a tape measure, which is the length from the root stem junction to the growth point. Measuring scion stem thickness with a vernier caliper, which is the thickness at 1 cm above the grafting junction parallel to the cotyledon development direction. Manually counting the number of leaves, starting from the bottom and counting them one by one, each time confirming the position of the previous leaf to avoid duplication or omission. A portable chlorophyll meter (SPAD-502) was used to measure the relative chlorophyll content of grafted seedlings. The determination of antioxidant enzyme activities (SOD, POD, and CAT) was carried out in a physiological laboratory, following the method of Nakano and Asada (1981) with slight improvements. The purpose of this study was to compare the differences in plant growth and antioxidant enzyme activity of grafted cucumbers, and to screen for good cucumber rootstock materials, providing a basis for the breeding and utilization of cucumber grafted rootstocks. This study used the membership function value method to analyze the comprehensive evaluation of the effect of pumpkin rootstock hybrid combination grafting on cucumber growth. Based on the research results, it was found that the Yanbian-3×360-3 grafted seedlings had the highest plant height, stem thickness, chlorophyll content, leaf number, and antioxidant enzyme activity indicators, with a total membership function value of 3.734, which was the highest. This indicates that, as a rootstock for grafting, it can improve the growth and antioxidant capacity of cucumber seedlings. Therefore, it can be validated and promoted as an excellent rootstock for cucumber grafting in production, providing a basis for screening excellent commercial cucumber rootstocks
To Cite this article:
Chen, R., V. Dubovyk, B. Chen and J. Liu. 2025. Comparison of the effects of seven hybrid combinations of pumpkin root stocks on the growth and antioxidant enzyme activity of grafted cucumber. Pak. J. Bot., 57(3): DOI: http://dx.doi.org/10.30848/PJB2025-3(37)
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