PJB-2024-535
Regulatory networks and metabolite variations during leaf growth and development in Drynaria roosii Nakaike
Hongyu Chen, Bo Wang, Ying Yu and Qingwen Sun
DOI:10.30848/PJB2025-5(38)
Abstract
Leaf development is a pivotal stage in the plant life cycle, involving intricate physiological and morphogenetic processes characterized by dynamically shifting molecular mechanisms and metabolite patterns, which have not been extensively studied in the traditional medicinal plant Drynaria roosii Nakaike. This study utilized next-generation sequencing to profile the leaf transcriptome of D. roosii, identifying significant differences in gene expression between two distinct developmental stages, with a focus on transcription factors that regulate leaf formation and metabolite production. De novo assembly of high-quality sequences generated 82,476 unigenes of average length 1,292 bp. FPKM analysis unveiled substantial transcriptome changes during leaf development. Additionally, non-targeted metabolomics detected 1,297 compounds across both stages, with lipids representing the most abundant metabolite group. This study offers dynamic insights into transcriptomic and metabolomic shifts during D. roosii leaf development, elucidates crucial regulatory networks involved in this process, and enhances the annotation of the D. roosii genome, paving the way for future investigations
To Cite this article:
Chen, H., B. Wang, Y. Yu and Q. Sun. 2025. Regulatory networks and metabolite variations during leaf growth and development in Drynaria roosii Nakaike. Pak. J. Bot., 57(5): DOI: http://dx.doi.org/10.30848/PJB2025-5(38)
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