PJB-2007-200
INDUCTION AND REGENERATION OF HYPOCOTYL DERIVED CALLI IN HOT CHILLI (CAPSICUM FRUTESCENS L.) VARIETIES
RABBIA HASNAT, NADEEM AKHTAR ABBASI, TOUQEER AHMAD AND ISHFAQ AHMAD HAFIZ
DOI:
Abstract
An efficient and reliable In vitro protocol was established for induction and regeneration of calli in varieties of hot chilli (Capsicum frutescens L.) var. Nepali and NARC-IV. Early initiation and induction with sustainable calli growth in both varieties was achieved on Murashige & Skoog medium supplemented with 1.5 mg l-1 of 2,4-D. Better performance in both varieties regarding shoot initiation, regeneration rate (%) and number of lateral shoots per regenerants were achieved on medium containing 3.0 mg l-1 BA. Rooting was achieved on half strength MS basal medium containing 1.0 mg l-1 IBA. Plantlets exhibiting normal morphology with extensive rooting were acclimatized in greenhouse.
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