PJB-2010-96
DEVELOPMENT OF AN EFFICIENT REGENERATION PROTOCOL FOR THREE GENOTYPES OF BRASSICA JUNCEA
RAISA BANO1,2, MOHAMMAD HAROON KHAN1,2, RAHAM SHER KHAN1, HAMID RASHID2* AND ZAHOOR A. SWATI1
DOI:
Abstract
Two phytohormones, auxins (Naphthalene acetic acid and Indole acetic acid) and cytokinins (Benzylaminopurine and Kinetin) with concentrations were used to develop an efficient regeneration protocol for 3 genotypes of Brassica juncea (UCD-635, RL-18 and NIFA RAYE). The explants were cultured on MS-medium supplemented with BAP 1.0 mgL-1/NAA 0.1 mgL-1, BAP 2.0 mg L-1/NAA 0.2 mg L-1, BAP 3.0 mgL-1/NAA 0.3 mg L-1 and Kinetin 1.0 mg L-1/IAA 0.1 mg L-1, Kinetin 2.0 mg L-1/IAA 0.2 mg L-1, Kinetin 3.0mg L-1/IAA 0.3 mg L-1. Maximum callus production (65.55) was observed on MS medium containing with BAP 2.0 mgL-1/NAA 0.2 mg L-1. Maximum shooting ( 22.31) was observed BAP 3.0 mg L-1/NAA 0.3 mg L-1 and KIN 3.0 mg L-1/IAA 0.3 mg L-1. Regeneration efficiency was found maximum (7.13) with BAP 3.0 mg L-1/NAA 0.3 mg L-1. The three genotypes were found significantly different at p≤0.05 in shoots production and regeneration efficiency.
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