PJB-2013-107
CHROMOSOME LOCATION AND SSR MARKERS OF A STRIPE RUST RESISTANCE GENE FROM WHEAT (TRITICUM AESTIVUM) LINE N9738
BAOJU YANG1, WANQUAN JI1*, CHANGYOU WANG1*, FEI XUE1, M. ALI2, YAJUAN WANG1, HONG ZHANG1 AND XINLUN LIU1
DOI:
The objective of present study here was to determine the inheritance and chromosomal location of the resistance gene in N9738. The phenotypic data of F2 plants, from the crosses N9738/Huixianhong and N9738/Abbondanza, respectively, implied that the stripe rust resistance in N9738 was controlled by a single dominant gene, temporarily designated YrN9738. Monosomic (Nullisomic) analysis revealed YrN9738 is on wheat chromosome 1B. Seventy-eight SSR markers on chromosome 1B were screened on the parents and bulks. Four SSR markers (Xgpw7422, Xgpw5195, Xgpw7812, and Xcfd65) were used to construct a linkage map for YrN9738 and Xcfd65 was closely linked to YrN9738 with genetic distance of 3.5 cM. These linked markers were located on chromosome 1BL with Chinese Spring nullisomic-tetrasomics and ditelosomics of homoeologous group 1. Their presence in the deletion stocks of chromosome 1B located Xgpw7422 and Xgpw7812 in bin 1BL06-0.32-0.47 and Xgpw5195 in bin 1BL06-0.32. YrN9738 should be on wheat chromosome 1B near to the centromere and useful in wheat breeding programs.
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