PJB-2003-5
MICROPROPAGATION OF POLIANTHUS TUBEROSA (TUBEROSE) THROUGH CALLUS FORMATION
S. NAZNEEN, MUSSARAT JABEEN AND IHSAN ILAHI
DOI:
Abstract
Callus was induced from bulb pieces and in vitro formed shoot buds in Polianthus tuberosa Willd., on Murashige & Skoog's (MS) medium supplemented with BAP and 2,4-D. Maximum callus induction from bulb pieces was 70% at 0.5 mg/l each of BAP and 2,4-D. Shoot buds showed 100% callus induction at 1.0 mg/l each of BAP and 2,4-D. Shoot regeneration response was observed only in buds derived callus. Highest number of shoots were regenerated from the callus at 3.0 mg/l of BAP. Best root development was achieved on MS medium supplemented with 0.5 mg/l NAA. Plantlets obtained were successfully transferred to the field with 100% survival rate.
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