PJB-2004-67
PLANT REGENERATION BY SOMATIC EMBRYOGENESIS FROM CALLUS OF MATURE SEED EXPLANTS OF BREAD WHEAT (TRITICUM AESTIVUM L.)
SAAD IMRAN MALIK1, HAMID RASHID2, TAYYABA YASMIN AND NASIR MAHMOOD MINHAS
DOI:
Abstract
The objective of this study was to test genotypes and to evaluate media for effectiveness in promoting whole plant regeneration by somatic embryogenesis in two wheat genotypes viz., Inqilab-91 and Pavon-76. Embryogenic callus was obtained by culturing seeds of both varieties on gel-solidified Linsmaier and Skoog's (LS) medium with 3.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D). The callus cultures were maintained by subsequent subcultures onto fresh media at 4-5 weeks interval. The embryogenic calli carefully cleared of any surrounding non-embryogenic callus were grown on the gel-solidified basic Murashige and Skoog's (MS) medium with 3% (w/v) sucrose after at least two subcultures. Seven different combinations of BAP (6-benzylaminopurine) and IAA (Indole-3-acetic acid) i.e., 0 & 0.1, 0.5 & 0.1
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