PJB-2018-1107
Epigenetic role of DNA methylation in hybrid vigor of cytoplasmic male sterility in sunflower
Omar Awad Kanoosh, Ayoob Obaid Alfalahi, Francis Oraha Jano and Hussam Fahim Najeeb Alawadi
DOI:10.30848/PJB2021-1(29)
Abstract
The molecular epigenetic relationship between DNA methylation and hybrid vigor using different sources of cytoplasmic male sterility of sunflower (six A-lines, three R-lines, and their 18 F1's) has been investigated. Molecular analysis of DNA methylation using methylation-sensitive amplification polymorphism (MSAP), revealed that the used four primers succeeded in characterizing a total of 36 specific loci, 35 out of these loci found to be methylation-susceptible (MSL). Meanwhile, only 27 loci were polymorphic achieving were 77% polymorphism percentage of 77%. Differently methylated regions (DMRs) indicated a large convergence between A-lines and single hybrid populations, whereby the percentage of unmethylated state reached 23% and 22% in both populations, respectively. The highest percentage of non-methylated state (31%) was detected in R-lines, whereas the hybrids gave the highest percentage of hemimethylated state (13%) against their sterile maternal lines (A-lines) and fertility restorer lines (R-lines) which were 10% and 8%, respectively. A-lines showed the minimum percentage of the internal cytosine methylation (10%), where as, the parental testers and the resulted hybrids gained a slightly higher different percentage (14% and 13%, respectively). The epigenetic distinctness of sterile A-lines was clear as they showed the highest value for full methylation state (57%) against testers and diallel hybrids (46% and 50% respectively). Hybrids like A3XR2 showed a unique methylation pattern to occupy a single cluster. Hybrids with higher hybrid vigor (A2XR1, A4XR2, and A6XR1) gave the highest positive heterosis values for plant yield
To Cite this article:
Kanoosh, O.A., A.O. Alfalahi, F.O. Jano and H.F.N. Alawadi. 2021. Epigenetic role of DNA methylation in hybrid vigor of cytoplasmic male sterility in sunflower. Pak. J. Bot., 53(1): DOI: http://dx.doi.org/10.30848/PJB2021-1(29)
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