PJB-2019-207
Rapid in-vitro propagation of Chrysanthemum morifolium through shoot bud explants
Muhammad Imtiaz, Abdul Mateen Khattak, Muhammad Ali Khan, Fazal Jalal, Sayed Hussain, Fazal Said and Hong Bo
DOI:10.30848/PJB2019-3(11)
Abstract
Chrysanthemum (Chrysanthemum morifolium) is perennial short day flowering plant. Nodal explants of Chrysanthemum were cultured on simple Murashige and Skoog (MS) media, MS media supplemented with different concentrations of 6-Benzyl adenine (6-BA) alone and in combination with different concentrations of Naphthalene acetic acid (NAA) or Thidiazuron (TDZ) for shoot initiation and the regenerated shoots were sub-cultured on MS, ½ MS and ¼ MS media for rooting. No shoot induction was recorded in explants cultured on simple MS, but the phyto-hormones addition to MS media resulted in 100% shoot induction. In case of MS media supplemented with 6-BA, maximum shoot buds (12) and shoot length (6.06 cm) explant-1 were recorded when 44.39 µM 6-BA was added to MS media. In case the MS media was supplemented with 6-BA+NAA, maximum (11)shoot buds explant-1 were recorded in explants cultured in MS+88.79 µM 6-BA+10.74 µM NAA media. Among the different concentrations of 6-BA+TDZ added to MS media, maximum (6.67) shoot buds explant-1 were recorded for explants planted in MS+88.79 µM 6-BA+9.08 µM TDZ. The regenerated shoots were then sub cultured on MS, ½ MS and ¼ MS media for rooting. In that case, there was non-significant effect on root length. However, maximum (15.78) roots were recorded for those cultured in ½ MS media. Thus it is concluded that supplementing MS media with 44.39µM 6-BA is the best concentration for rapid shoot regeneration and½ MS is optimal for the In vitro rooting of Chrysanthemum morifolium.
To Cite this article:
Imtiaz, M., A.M. Khattak, M.A. Khan, F. Jalal, S. Hussain, F. Said and H. Bo. 2019. Rapid in-vitro propagation of Chrysanthemum morifolium through shoot bud explants. Pak. J. Bot., 51(3): DOI: http://dx.doi.org/10.30848/PJB2019-3(11)
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