Pak. J. Bot., 37(1): 155-161, 2005. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
A SURVEY OF AMYLOLYTIC BACTERIA AND FUNGI FROM NATIVE ENVIRONMENTAL SAMPLES MUHAMMAD SOHAIL, AQEEL AHMAD, SALEEM SHAHZAD1 AND SHAKEEL AHMED KHAN*
Abstract: Starches are one of the most important naturally occurring glucose
polymers that are present in plants. They are used as the starting material
for the production of a variety of different types of compounds of commercial
importance. The usual process is the treatment of starch at an elevated
temperature to gelatinize followed by liquefaction by chemicals or by
enzymes. Enzymatic liquefaction has an edge over chemical treatment
process. Microbes are, in general, the source of commercial enzymes.
Present study was initiated to screen the microbial population for their
ability to possess amylolytic potential where 117 strains of bacteria
and 130 fungal strains were isolated and screened. A considerable number
of both were found to produce amylase. Bacillus sp., and Aspergillus
sp., were the most active amylase producers. Bacterial enzymes showed
maximum activity at slightly elevated temperature and at alkaline pH
while one of the fungal enzymes retained most of its activity even at
a temperature of 80ºC, however, level of activity was reduced when
reaction was carried out at alkaline pH. Genetic studies revealed that
starch is a suitable inducer in case of fungi, while maltose seems to
be a better inducer than that of starch in case of bacteria. Department of Microbiology, University of Karachi, Karachi-75270, Pakistan. Department of Botany, University of Karachi, Karachi-75270, Pakistan. |
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