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Pak. J. Bot., 46(4): 1445-1452, 2014.

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METABOLOMIC VARIATION OF BRASSICA RAPA VAR. RAPA (VAR. RAAPSTELEN) AND RAPHANUS SATIVUS L. AT DIFFERENT DEVELOPMENTAL STAGES

MUHAMMAD JAHANGIR*^1,5, IBRAHIM BAYOUMI ABDEL-FARID^2,5,6, RIC C. H. DE VOS^3,4, Harry H. Jonker^3,4, YOUNG HAE CHOI⁵ AND ROBERT VERPOORTE⁵

Abstract: Brassica rapa (var. raapstelen) and Raphanus sativus (red radish) are being used as food and fodder while also known as model in recent plant research due to the diversity of metabolites as well as genetic resemblance to Arabidopsis. This study explains the change in metabolites (amino acids, organic acids, chlorophyll, carotenoids, tocopherols, ascorbic acid, sucrose, phenylpropanoids and glucosinolates) during plant development. In present study the metabolomic variation in relation to plant growth has been evaluated, for Brassica rapa (var. raapstelen) and red radish (Raphanus sativus) at three different developmental stages. A non-targeted and targeted metabolomic approach by NMR and HPLC in combination with Principal component analysis (PCA) of the data was used to identify phytochemicals being influenced by plant growth. The results lead to the better understanding of metabolic changes during plant development and show the importance of plant age with respect to the metabolomic profile of vegetables.

¹Section of Food Science & Technology, Department of Agricultural Sciences, University of Haripur, Pakistan

²Aswan University, Faculty of Science, Botany Department, 81528 Aswan, Egypt

³Plant Research International, POB 16, 6700 AA Wageningen, the Netherlands.

⁴Centre for BioSystems Genomics, P.O. Box 98, 6700 AA Wageningen, the Netherlands.

⁵Natural Product Lab, Institute of Biology, Leiden University, Leiden, The Netherlands

⁶Aljouf University, Faculty of Science, Biology Department, Sakaka, KSA

*Corresponding author e-mail: mj_awan@hotmail.com; 0092-995-513878; 0092-995-615393