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Year 1994 , Volume  26, Issue 1
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S.No. Title Authors Pages Download
1
DR. R. R. STEWART (1890-1993)
S. I. ALI AND A. GHAFFAR

DR. R. R. STEWART (1890-1993)
ABSTRACT:
Dr. Ralph Randles Stewart died on 6th November, 1993 at the age of 103 years at Duarte, Calif., USA. May God Almighty shower His blessings upon the departed soul and may his soul rest in peace in heaven. Amen. Dr. Ralph Randles Stewart was born on 15th April 1890 and grew up at Manhattan Island, New York city, USA. He obtained his Ph.D. degree (1916) from Columbia University, New York; D.Sc. Honorary (1953) from the University of the Punjab, Lahore and LLD Honorary (1963) from Alma College, Michigan, USA.

1-2 Download
2
DR. SYED FAKHRUL HASSAN (1924-1994)
ABDUL GHAFFAR

DR. SYED FAKHRUL HASSAN (1924-1994)
ABSTRACT:
Dr. Syed Fakhrul Hassan died of cardiac arrest due to respiratory failure on 1st March 1994. May God Almighty shower his choicest blessings upon the departed soul and may his soul rest in peace in heaven. Amen.

3-4 Download
3
DR. GHULAM RASOOL SOLANGI (1938-1993)
MASHOOD AHMED SIDDIQUI

DR. GHULAM RASOOL SOLANGI (1938-1993)
ABSTRACT:
Dr. Ghulam Rasool Solangi S/O Mr. Alam Khan Solangi was born on 6th December, 1938 at Larkana, Sindh. After obtaining early education from his home town, he studied at the Sindh Agriculture College, now Sindh Agricultural University, Tandojam from where he graduated in Agriculture in 1962. He received his M.Sc degree in Plant Pathology from the Sindh University, Jamshoro in 1965. In 1968 he went to USA and worked with Prof. H.K.Kiesling at the North Dakota State University, Fargo, U.S.A. He was awarded the degree of Ph.D in 1973 for his research studies on the "Genetic systems in Cochliobolus sativus".

5-6 Download
4
NODULATION STATUS OF LEGUMINOUS PLANTS IN SINDH
A. MAHMOOD AND PARVAIZ IQBAL

NODULATION STATUS OF LEGUMINOUS PLANTS IN SINDH
ABSTRACT:
A survey of 115 leguminous plants comprising of 79 species of Papilionaceae, 16 of Mimosaceae and 20 of Caesalpiniaceae was made for their nodulation status. Nodules were found in all the species of Papilionaceae, all the species of Mimosaceae except Adenanthera pavonina but not in Caesalpiniaceae. Vigna dalzelliana (O. Kuntze) Verde., V. mimgo (L) Hepper var. Sialkot; V. radiata (L) Wilezek. Pak.17. V. radiata (L.) Wilczek var. 71-17, V. radiata (L) Wilczek var. 3854, V. radiata (L) Wilczek var. 6601, V. Utlguiculata (L.) Walp. Tvx-O2F.3871 and V. unguiculata (L) Walp 1182 E-13 of the family Papilionaceae are reported for the first time as nodule bearing. Caesalpinia gilliesii (Hook) Dietr, Cassia italica(Mill). F.P. Andr, ssp.italica and C. italica ssp. micrantha Brenan of the family Mimosaceae are reported for the first time as non-nodulating. The shapes of'nodules were mainly elongate, globose and branched whereas semiglobose and irregular shapes were also observed. The colour of the nodules varied from white to brown and pink. Pink coloured nodules were mainly associated with members of Papilionaceae.

7-20 Download
5
A PALYNOLOGICAL STUDY OF MONO COTS FROM KARACHI (EXCLUDING GRAMINEAE)
SEEMA SULTAN, ANJUM PERVEEN AND M. QAISER

A PALYNOLOGICAL STUDY OF MONO COTS FROM KARACHI (EXCLUDING GRAMINEAE)
ABSTRACT:
Pollen morphology of 25 monocotyledonous species belonging to 14 genera distributed in 8 families from Karachi have been investigated by light and scanning electron microscope. Pollen occur either singly or in tetrads. In most of the species simple apertures are found i.e., colpate and porate. However, in some cases iII-defined apertures or no apertures as in Commelinaceae are also found. Tectum of these families are also quite variable which ranges from reticulate, rugulate, striate, fossulate- echinate or scabrate/areolate.

21-34 Download
6
POLLINATION TYPES AND POLLEN CHARACTERS WITHIN FLORA OF KARACHI
ANJUM PERVEEN, TAHIR ALl AND M. QAISER

POLLINATION TYPES AND POLLEN CHARACTERS WITHIN FLORA OF KARACHI
ABSTRACT:
The correlation between pollen characters and pollination types in 218 genera and 351 species belonging to 67 angiosperm families from Karachi was studied. Entomophily is the dominant (65.24%) pollination type, while 33.04% taxa are anemophilous and about 1.71 % taxa altogether exhibit ornithophily, hydrophily and zoophily. A definite relationship occurs between pollen characters and pollination types especially in entomophily and anemophily. Pollen grains of entomophilous taxa are characterized by compound apertures i.e., 3-colporate, prolate-spheroidal shape, generally large, thick walled, sticky and with reticulate tectum, while pollen grains of anemophilous taxa are with simple apertures i.e., monoporate, spheroidal, small, thin walled, dry and with scabrate-areolate tectum.

35-56 Download
7
NUMERICAL ANALYSIS OF THE GENUS POGOSTEMON DESF. (LAMIACEAE)
MAHBUBA KHANAM, ALASTAIR CULHAM, SYED Z.HUSAIN AND SYEDA S. TAHIR*

NUMERICAL ANALYSIS OF THE GENUS POGOSTEMON DESF. (LAMIACEAE)
ABSTRACT:
Numerical phenetic analysis of Pogostemon Desf. (Lamiaceae) species was carried out using a Reading Taxonomy Computer Programme. Thirteen species found in Bangladesh were analyzed for 34 characters. Three Phenograms derived from nearest neighbour, furthest neighbour and group average clustering were produced. The results obtained are discussed in the light of previous classification.

57-62 Download
8
MEIOTIC CHROMOSOME CONFIGURATIONS IN RHOEO SPATHACEA (SWARTZ) STEARN
BUSHREEN JAHAN AND AHSAN A. VAHIDY

MEIOTIC CHROMOSOME CONFIGURATIONS IN RHOEO SPATHACEA (SWARTZ) STEARN
ABSTRACT:
Rhoeo spachacea exhibits uncommon and unique chromosomal configurations at diakinesis and metaphase I in the form of rings or chains. Chain configurations range from a chain of two to twelve chromosomes with every intermediate size in one, two or three groups. A chain of twelve chromosomes was the common configuration than ring. In the case of two chains per cell highest frequency (10.71 %) was observed with 7+5 chromosomes and lowest (4.28%) with 10+2 configuration. Different configurations of three chains per cell were found in 0.7-4.28% cells. However by comparisons of chromosome configurations in R spathaceastudied by different workers significant variations were observed. These variations could be due to the use of different clones of Rhoeo or to different horticultural environments.

63-67 Download
9
CYTOTOXIC EFFECTS OF HERBICIDE RON STAR ON MERISTEMATIC CELLS OF ALLIUM CEPA L
SADIA K. BUTT AND AHSAN A. V AHIDY

CYTOTOXIC EFFECTS OF HERBICIDE RON STAR ON MERISTEMATIC CELLS OF ALLIUM CEPA L
ABSTRACT:
Actively growing root tips of onion (Allium cepa L) were used to evaluate cytotoxic effects of herbicide, Ronstar. The dividing cells exposed to 1000, 2000, 3000 and 4000 ppm of Ronstar for 3, 6, 9 and 12 h durations showed a significant reduction in mitotic index especially in treatments of higher concentrations and longer durations. Stickiness and fragmentation of chromosomes were the most frequent aberrations observed, bi- and multi-nucleate cells were noted as well. Abnormalities in lesser frequencies included Cmitosis and polyploidy. Such chromosomal abnormalities may effect adversely the vigor, fertility, yield or competitive ability of the plants exposed to the herbicide.

69-74 Download
10
HERITABILITY ESTIMATES AND CORRELATION STUDIES OF EARLY MATURITY AND OTHER AGRONOMIC TRAITS IN TWO CROSSES OF PEANUT (ARACHIS HYPOGAEA L)
NAAZAR ALl AND J.C. WYNNE*

HERITABILITY ESTIMATES AND CORRELATION STUDIES OF EARLY MATURITY AND OTHER AGRONOMIC TRAITS IN TWO CROSSES OF PEANUT (ARACHIS HYPOGAEA L)
ABSTRACT:
 Knowledge of heritability and correlation among traits is important in determining the effectiveness of direct and indirect selection. The objective of this study was to determine the potential effectiveness of selection for early maturity, and seed size, in two peanut crosses (NC 7/Chico and 73-30/Chico) byestimating the heritability for each trait and the correlations among the traits. Both narrow sense heritability and broad sense heritability estimates were fairly high for seed weight, maturity index, and pod length in both the crosses. Broad-sense heritability estimates were much greater than parent-offspring regressions and were biased upward due to nonadditive genetic effects and genotype x environment interaction. The results suggested that selection for early maturity, seed weight, and pod length could be practiced in early segregating generations. Correlations of maturity with shelling percentage were positive and highly significant in both crosses but correlation between maturity and seed number was positive and significant in cross 1 (NC 7/Chico). Maturity was negatively correlated with pod length and seed weight in cross 2. The highest positive and significant correlation was found between pod length and seed weight.

75-82 Download
11
HYBRIDIZATION IN SIDA OVATA COMPLEX II. EVIDENCE FROM BREEDING STUDIES
RUBINA DAWAR, TAHIR ALl AND M. QAISER

HYBRIDIZATION IN SIDA OVATA COMPLEX II. EVIDENCE FROM BREEDING STUDIES
ABSTRACT:
Breeding studies reconfinned the hybridization between Sida avata Forssk., and Sida tiagii Bhandari. All the three taxa i.e., S. avata Forssk., S. tiagii Bhandari and the hybrid are facultative autogamous as indicated by pollen - ovule ratios and breeding experiments. Chrysis sp., (Hymenoptera) seems to be responsible for pollen transfer between S. avata and S. tiagii in natural populations. Certain insect pollinators were specific such as Bembix sp., and Bambus sp., for S. avata, Apis sp., and Vespa sp., for S. tiagii and Ponera sp., for the hybrid. The limited number of hybrids may be due to less frequent visitation of common pollinator (Chrysis sp.) alongwith the incomplete reproductive barder between both the parents. The restricted gene flow is also evident by least fruit and seed set in hand pollinated reciprocal crosses of the putative parents as compared to the self pollination. It seems that back crosses are impeded due to the absence of common pollinators between the parents and hybrid and meiotic irregularities as indicated by the failure of hand pollinated (back crosses) experiments.

83-97 Download
12
DEVELOPMENT OF A HIGH YIELDING COTTON MUTANT, NIAB-92 THROUGH THE USE OF INDUCED MUTATIONS
R.M. SAEED IQBAL, M.B. CHAUDHRY, M.ASLAM AND A.A. BANDESHA

DEVELOPMENT OF A HIGH YIELDING COTTON MUTANT, NIAB-92 THROUGH THE USE OF INDUCED MUTATIONS
ABSTRACT:
Soaked seeds of an exotic variety 5toneville-231 (Gossypium hirsutum L) were treated with gamma rays at 30 kR from 60CO source having dose rate of 40 kR per hour and planted in the field as M1 genera fion during 1984. From the segregating generations, a high yielding mutant was selected and named as NIAB-92. The plant of NIAB-92 is a semi-haily, compact sympodial type of medium stature. It has 0-2 fruit bearing monopodial branches and more number of shorter sympodial branches as compared to the parent, Stoneville-231 and commercial cotton variety NIAB-78. The mutant is early maturing and matures twenty days earlier than the parent 5toneville-231. It has higher yield potential alongwith desirable fibre properties such as G.O.T.(%), fibre length, fibre fineness and fibre strength. In the comparative yield trials the mutant NIAB-92 significantly outyielded both the prevalent commercial cotton varieties i.e., NIAB- 78 and S-12. At NIAB it gave 15.3 % and 19.1 % higher yield than NIAB-78 and 5-12, respectively, while at farmers fields the increase in yield was 8.8 % and 14.1 % as compared with NIAB-78 and 5-12. The mutant has also shown resistance against leaf curl virus disease.

99-104 Download
13
PLANT GROWTH AND IONIC DISTRIBUTION IN COTTON (GOSSYPIUM HIRSUTUM I..) UNDER SALINE ENVIRONMENT
ALl ZAFAR JAFRI* AND RAFIQ AHMAD

PLANT GROWTH AND IONIC DISTRIBUTION IN COTTON (GOSSYPIUM HIRSUTUM I..) UNDER SALINE ENVIRONMENT
ABSTRACT:
Experiment was conducted on four cotton (Gossypium hirsutum L) cultivars viz., B.557, Niab-78, Sar. mast, Qalandari to evaluate their growth in terms of plant height, stem diameter, biomass production and ion transport from root to shoot when exposed to salinity levels of 4, 16, 21, 22 and 24 dS, m-1. Increasing salinity levels reduced growth of all the cultivars with higher inhibition in shoot as compared to root, Cw, Niab 78 and Qalandari exhibited higher salt tolerance where lower salinity levels promoted root development. Increasing salt levels also increased the net ionic uptake in all the cultivars with more affinity for K +. KINa ratio was > 1 in all cultivars, Niab- 78, however, showed minimum uptake and accumulation of salts under increasing salinity regimes and can be rated as more salt tolerant.

105-114 Download
14
RESPONSES OF SPOROBOLUS ARABICUS AND SESBANIA AEGYPTICA AS AFFECTED BY DENSITY, SALINITY OF IRRIGATION WATER AND INTER-CROPPING
RAFIQ AHMAD AND SYED HASAN ZAHEER

RESPONSES OF SPOROBOLUS ARABICUS AND SESBANIA AEGYPTICA AS AFFECTED BY DENSITY, SALINITY OF IRRIGATION WATER AND INTER-CROPPING
ABSTRACT:
Sporobolus arabicus and Sesbania aegyptica grown in sandy soil as mono- and mix-culture using saline water (ECiw: 6 & 12 dS.m-1) for irrigation showed that the productivity of grass - legume mix-culture per unit area was much higher irrespective of the salinity of irrigation water. Reduction in growth of S. aegyptica at higher salinty (ECiw: 12 dS.m-1) was more as compared to S. arabicus. No significant reduction in growth of S. aegyptica was observed at low salinity (ECiw: 6 dS.m-1), whereas growth in S. arabicus was promoted. Increase in proline content at higher salinity was evident in S. aegyptica with no effect in S. arabicus. Alteration in the absorption of radiant energy (quantum yield) were non-significant in Sporobolus in response to salinity and cropping system, while Sesbania plants showed a positive response with respect to salinity and inter-cropping with Sporobolus. The same trend was evident for stomatal conductance. Transpiration rate of Sporobolus was low when grown as mix-culture, while that of Sesbania was highly reduced by salinity under both cropping systems.

115-125 Download
15
EFFECT OF SALT AND WATER STRESS ON GROWTH AND BIOMASS PRODUCTION IN HELIANTHUS ANNUUS L.
SHAZIA HUSAIN AND SHOAIB ISMAIL

EFFECT OF SALT AND WATER STRESS ON GROWTH AND BIOMASS PRODUCTION IN HELIANTHUS ANNUUS L.
ABSTRACT:
The effect of 0.25-1.0% NaCI salinity and water stress on growth and biomass production in Helianthus annuus L, was examined. All growth parameters showed reduction under both salt and salt water stress. One percent NaCI salinity adversely affected the relative growth rate (RGR), net assimilation rate (NAR) and leaf area ratio (LAR). Salt stress together with moisture stress also adversely affected the growth rate. Plants grown both under salt or salt and water stress conditions showed a better growth response after 1 month interval followed by a decline at subsequent harvests.

127-138 Download
16
ALLELOPATHIC POTENTIAL OF ALBIZIA SAMANS MERR.
MEHER NOOR AND M. AJMAL KHAN

ALLELOPATHIC POTENTIAL OF ALBIZIA SAMANS MERR.
ABSTRACT:
The effect of aqueous extracts obtained from various plant parts of Albizia samons and the under canopy soil was studied on germination and early seedling growth of different cultivars of Zea mays, Triticum aestivum and Albizia lebbeck. Stem and seed extracts delayed germination and inhibited seedling growth. Root and leaf extracts delayed germination and inhibited seedling growth in some test species. Rower and soil extracts had no effects on germination and growth.

139-147 Download
17
PHYTOSOCIOLOGY OF THE VANISHING TROPICAL DECIDUOUS FOREST IN DISTRICT SWABI, PAKISTAN. II-ORDINATION
FARRUKH HUSSAIN, MOINUDDIN AHMED1, GHAZALA SHAHEEN2 AND MUHAKHARA JAN DURRANI2

PHYTOSOCIOLOGY OF THE VANISHING TROPICAL DECIDUOUS FOREST IN DISTRICT SWABI, PAKISTAN. II-ORDINATION
ABSTRACT:
The study deals with the multivariate analysis of the vegetation of Swabi District. Sociological relationships among the leading dominants in tree, shrub and herb layers are discussed separately. Chcmical and physical analysis of soils of each stand are given. The vegetation pattern of each layer of the community and its relationship to soil were examined with the help of polar ordination. Soil pH, CaCO3 and P2O5 were found to be the controlling factors in the distribution of vegetation. The overall vegetation is dominated by Acacia modesta, followed by Ziziphus mauritiana in the tree layer. Asparagus, lmodon, Torilis, Thymelaea, Qxalis and Desmostachya were important in the lower strata.

149-160 Download
18
EFFECTS OF STORAGE TEMPERATURE ON GROWTH AND BULB FORMATION IN FOUR GARLIC (ALLIUM SATIVUM L) CULTIVARS
S.M.E. SATTI AND M. LOPEZ

EFFECTS OF STORAGE TEMPERATURE ON GROWTH AND BULB FORMATION IN FOUR GARLIC (ALLIUM SATIVUM L) CULTIVARS
ABSTRACT:
Studies on the effect of storage temperature of 0,7 and 25°C before planting were conducted on four garlic cultivars with respect to their leaf initiation, clove differentiation, development and yield under field conditions during 1990/91 season. All cultivars, except Omani, showed less leaf initation at 25°C than at either 0 or 7°c. In contrast, Omani garlic produced more leaves at 25°C than at lower storage temperatures. A varied cultivar response to temperature was noticeable in leaf development over a 15 weeks period from planting. Bulb diameter was greater at low temperatures. Clove differentiation was higher in Omani cultivar and least in Indian cuItivar. Single clove bulbing was found to increase with temperature, reaching .80% at 25°c. Bulb weight and number of days to reach maturity were both favoured by low storage temperature. Bulb yield showed progressive decrease with the increase in storage temperature. However. all cultivars, except Indian, attained a 3 fold increase in bulb yield at OOC than at 25°C.

161-165 Download
19
INFLUENCE OF TOPPING RAPESEED ON YIELD COM PONENTS AND OTHER AGRONOMIC CHARACTERS UNDER VARYING DATES OF PLANTING
RAHMAT ULLAH KHAN, H.H. MUENDEL* AND M. FAYYAZ CHAUDHRY

INFLUENCE OF TOPPING RAPESEED ON YIELD COM PONENTS AND OTHER AGRONOMIC CHARACTERS UNDER VARYING DATES OF PLANTING
ABSTRACT:
The effects of different planting dates were studied to determine the influence of topping at different growth stages on yield components, seed weight, seed yield and other agronomic characters. The time of topping had a significant effect on initial flowering and maturity periods. More days were required for initial flowering and maturity for the ratooned crop topped at first flower followed by the bud and pre-bud stage except where the secondary branches were removed, which appeared to be almost similar to the controls. Topping, however, had a negative effect on yield components. Removal of secondary branches had minimum effect on yield components and produced yield per plant and plot very close to the controls whereas, topping response was clearly evident on seed weight.

167-171 Download
20
LEAF RUST DEVELOPMENT IN VARIOUS CULTIVARS OF WHEAT CARRYING DIFFERENT KNOWN GENES FOR RESISTANCE
A.A. HAKRO, M. ASLAM AND A.K. KHANZADA

LEAF RUST DEVELOPMENT IN VARIOUS CULTIVARS OF WHEAT CARRYING DIFFERENT KNOWN GENES FOR RESISTANCE
ABSTRACT:
Wheat cultivars Punjab 76 (Lr1), Chenab 70 (Lr13), Khyber 79 (Lr13), ZA-77 (Lr1 + 13), Faisalabad 83 (Lr+13), Lyallpur 73 (Lr1+13), Pari-73 (Lr1+13), Sandal (Lr1+13), Sindh 81 (Lr1 + 13). Yecora (Lr1 + 13), Zamindar (Lr1 + 13), Zarghoon (Lr1), Arz (Lr3 + 13), Barani 83 (Lr3 + 13), Punjab 81 (U13 + ) and Morocco (without gene) for leaf rust resistance were induded in the test. The plants 45 days after planting were inoculated through spraying with aqueous suspension of uredospores of Puccinia rccondita. Leaf rust epidemic was first observed early in February on Morocco which does not carry any known gene for resistance. The start of the disease on the cultivars carrying one or other gene (s) for resistance were delayed by one to three weeks. Similarly the rate of development of the disease per unit time was also slower on cultivars carrying a gene for resistance than on the one without any knwon gene for resistance.

173-176 Download
21
LOCATION OF MACROPHOMINA PHASEOLINAIN SEEDS OF PUMPKIN AND DEVELOPMENT OF CHARCOAL ROT DISEASE
NASREEN SULTANA, A.K. KHANZADA AND A. GHAFFAR*

LOCATION OF MACROPHOMINA PHASEOLINAIN SEEDS OF PUMPKIN AND DEVELOPMENT OF CHARCOAL ROT DISEASE
ABSTRACT:
Using component plating technique Macrophomina phaseolina was isolated from seed coat. tegmen!. cotyledons and embryo of pumpkin seeds. Infected seeds give rise to infected seedlings that transmit the pathogen into the fruits.

177-180 Download
22
BIOLOGICAL CONTROL OF SOILBORNE ROOT INFECTING FUNGI IN TOMATO AND OKRA
SHAHIDA PERVEEN, S. EHTESHAMUL-HAQUE* AND ABDUL GHAFFAR

BIOLOGICAL CONTROL OF SOILBORNE ROOT INFECTING FUNGI IN TOMATO AND OKRA
ABSTRACT:
In a green house experiment soil artificially infested with a pathogenic strain of Fusarium oxysporum f.sp lycopersici suppressed the infection of Macrophomina phaseolin a, F. solani and Rhizoctonia solani on tomato and okra roots. The suppressive effect of F. oxysporum increased in the presence of Trichoderma harzianum, T. koningii, Gliocladium virens, Paecilomyces lilacinus, Bradyrhizobium japonicwn and Rhizobium meliloti against F. solani and M. phaselina. F. oxysporum induced mortality in tomato plants. Root colonization by F. oxysporum increased in F. oxysporum infested soil with reduced plant growth and less infection of M. phaseolina and R solani. T. harzianwn, T. konillgii, G. virens, P. Iilacinus, B. japonicum and R meliloti showed significant control of M. phaseolina, F. oxysporum and F. solani infection on tomato and okra both in F. oxysporum infested and uninfested natural soil, whereas T. koningii, G. virens and B. japonicum increased R solaniinfection in F. oxysporum infested soil in tomato.

181-186 Download
23
THE REACTION AND THE INFLUENCE OF NITROGEN LEVELS ON INCIDENCE AND SEVERITY OF CURVULARIA LEAF SPOT DISEASE ON SUGARCANE (SACCHARUM OFFICINARUM L.)
E. O. SALAWU AND S. S. AFOLABI

THE REACTION AND THE INFLUENCE OF NITROGEN LEVELS ON INCIDENCE AND SEVERITY OF CURVULARIA LEAF SPOT DISEASE ON SUGARCANE (SACCHARUM OFFICINARUM L.)
ABSTRACT:
The reaction of sugarcane hybrids Co.440, Co.1oo1, Co.997, OB5155, CP.36/111, CP.291116, Mex 52/29, B61208, BR.6223, BO.20/58 to Curvularia leaf-spot disease was studied in the Screenhouse. Mex 52/29 and BN61208 were moderately resistant to infection while others were moderately to highly susceptible. Increase in nitrogen levels from 80 to 240 kglha increased the severity of attack. Sugarcane leaf necrotic spots coalesced, turned brown gradually and subsequently died, resulting into significant decrease in shoot weight of sugarcane hybrid Co.1oo1.

187-189 Download
24
PLANT REGENERATION FROM CARICA PAPAYA CV. MALIR GROWN IN TISSUE CULTURE
K. KHATOON AND R. SULTANA

PLANT REGENERATION FROM CARICA PAPAYA CV. MALIR GROWN IN TISSUE CULTURE
ABSTRACT:
Petiole segments and excised epidermal thin cell layers of Carica papaya N. Malir were cultured on full strength MS medium containing a range of concentrations of nephthalene acetic acid (NAA) and isopentenyl adenine (2iP) used seperately and in combination. Petiole segments and epidermal thin cell layers formed maximum callus on medium containing 2.5 mg/1 NAA plus 0.5 mgll2iP and 1 mg/1 NAA plus 1 and 5 mgll 2iP respectively. Enhanced callus growth and shoot formation occurred on half strength macro inorganic salts of MS medium containing 2.5 mg/1 NAA plus 0.5/1 mgll 2iP alongwith 15% coconut milk. Regenerated shoots produced roots on half strength macroinorgani<; salts containing MS medium supplemented with 5 g/1 glucose. The method may be useful for the mass propagation of female papaya plants for large scale cultivation.

191-195 Download
25
SOMATIC EMBRYOGENESIS FROM IMMATURE COTYLEDONS OF CHICKPEA (CICER ARIETINUM L.)
R. ISLAM

SOMATIC EMBRYOGENESIS FROM IMMATURE COTYLEDONS OF CHICKPEA (CICER ARIETINUM L.)
ABSTRACT:
Regeneration of plants by means of somatic embryogenesis is of utmost importance for improvement through somatic cell genetics. This is preferred over normal regeneration via organogenesis due to various advantages when used for transformation. High number of regenerants can be obtained originating from few or single cells which increases the likelihood of achieving transformed plants. Little progress has been made towards developing an in vitro regeneration system for chickpea, an important food legume crop. To date, chickpea has been regenerated only from preexisting shoot meristems (Rao & Chopra, 1989'; Rao & Reddy, 1992). This study describes the development of chickpea somatiic embryos on immature cotyledon explants.

197-199 Download
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