Current status and molecular characterization of Zucchini yellow mosaic virus (ZYMV) infecting ridge gourd (Luffa acutangula L.) in different regions of Punjab, Pakistan
Zohaib Asad, Muhammad Ashfaq, M. Inam-Ul-Haq, Gulshan Irshad and M. Azam Khan
Zucchini yellow mosaic (ZYMV) included in the genus Potyvirus is one of the most destructive pathogens of cucurbits, including ridge gourd (Luffa acutangula L.) which is widely grown in Indo-Pak subcontinent and can cause significant yield losses worldwide. In the present study, a total of 300 leaf and fruit samples of ridge gourd with virus-like symptoms were collected from 03 districts of Punjab, Pakistan. To perform an initial screening of ZYMV these samples were subjected to Plate Trap Antigen-Enzyme Linked Immunosorbent Assay (PTA-ELISA) using monoclonal antibodies. Overall disease incidence during 2018-2019 was 28.33%. The prevalence of ZYMV was confirmed in all ridge gourd sampling sites. ELISA-positive samples were further confirmed through RT-PCR and sequence analysis. Comparison of sequences with those available in Genbank showed 91-98% nucleotide and 98%-100% amino acid-based homology. Phylogenetic tree analysis revealed that Pakistani ZYMV ridge gourd isolates (MN897100, and MN897101) have close relationship with South Korean, Chinese and Turkish ZYMV isolates, and strengthened the belief that ZYMV Pakistani isolates reported in this study has Asian origin. Identification of new ZYMV isolates strengthen the breeding programs for the development of resistance genotypes to manage this notorious virus.
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