Hydrogen sulfide induced by hydrogen peroxide mediates darkness-induced stomatal closure in Arabidopsis thaliana
Yinli Ma, Liuxi Wang, Luhan Shao, Jiao Niu and Fengxi Zheng
Hydrogen sulfide (H2S) plays an important role in the regulation of stomatal movement in plants. Here we present the relationships and functions of H2S and hydrogen peroxide (H2O2) in stomatal movement by darkness in Arabidopsis thaliana. H2S synthesis inhibitors and scavengers inhibited darkness-induced stomatal closure, H2S generation and L-/D-cysteine desulfhydrase (L-/D-CDes) activity increase in wild-type leaves. Darkness induced stomatal closure in wild-type plants, but failed in L-/D-CDes deletion mutants Atl-cdes and Atd-cdes. Additionally, both L-/D-CDes activity and H2S content were significantly decreased after applying H2O2 synthesis inhibitors and scavengers, but there was almost no effects on H2O2 levels in the presence of H2S synthesis inhibitors and scavengers in wild-type leaves in darkness. Moreover, darkness couldn't increase H2S content and L-/D-CDes activity of mutant lines of NADPH oxidase gene AtrbohF and AtrbohD/F mutants leaves, but increased H2O2 levels in Atl-cdes and Atd-cdes guard cells. Taken together, we conclude that both H2S and H2O2 are involved in darkness-induced stomatal closure, and H2S functioned downstream of H2O2 in A. thaliana. Darkness induced H2O2 synthesis via promoting the activity of NADPH oxidase, and further led to the production of L-/D-CDes-derived H2S and stomatal closure in A. thaliana. NADPH oxidase gene AtrbohF participated in the process.
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