PJB-2020-275
Prediction of the Sea Buckthorn AQP Gene Structure and its Spatiotemporal Expression Pattern under Drought Stress
Dan Zhang
Abstract
Sea buckthorn has become a pioneer species in northwestern China due to its great ecological and economic value and its tolerance to extreme environments. Therefore, characterization of the drought resistance mechanism in sea buckthorn is of great significance for improving land desertification in semi-arid areas, breeding new drought-resistant varieties and developing and utilizing stress-resistant genetic resources. In this study, we used PCR amplification to amplify the sea buckthorn aquaporin (AQP) gene. The full-length AQP gene was obtained by RACE technology and sequenced. The secondary structure, three-dimensional structure and subcellular localization, transmembrane domain and functional sites of AQP were analysed, indicating that the AQP gene was distinct in sea buckthorn. The expression of AQP in different tissues was studied. The results showed that AQP was located to the cell membrane, and the main constituent elements of the protein secondary structure were composed of α-helixes and random coils. In addition, AQP was weak in hydrophilicity and contained seven transmembrane helices. The results of AQP gene expression analysis indicated that AQP was expressed in the roots, stems and leaves of sea buckthorn. AQP expression in each tissue reached the highest level on the 15th day after stress and decreased rapidly after rehydration. The above results indicate that the AQP gene has distinct structural characteristics and expression patterns to adapt to drought stress. This study provides the foundation for further characterizing the mechanism of sea buckthorn drought resistance.
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