PJB-2020-288
Transgenic Leymus chinensis expressing betaine aldehyde dehydrogenase and choline monooxygenase from Suaeda liaotungensis show different tolerance increased saline-alkaline
Tongbao Qu
Abstract
As an important osmotic adjustment protectant in response of plant to saline-alkali stress, glycine betaine (GB) is successively synthesized by choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH) in betaine-accumulating higher plants. Although many experiments showed that transgenic plant expressing CMO and/or BADH can increase saline-alkaline tolerance, it keep unclear in Leymus chinensis. In present study, different saline-alkaline tolerance of transgenic Leymus chinensis expressing CMO, BADH and CMO-BADH genes was studied. GB content of transgenic plants (CMO, BADH and CMO-BADH) significantly increased by 15.29%, 75.61% and 89.02%, and biomass increased by 128.13%, 252.84 and 265.91 at 200 mmol·L-1 Na2CO3-NaHCO3 stress compared with controls, respectively. The result showed that saline-alkali tolerance of transgenic plants was significantly increased, and exogenetic BADH might play a greater role than CMO in improving betaine content and saline-alkaline tolerance in transgenic L. chinensis.
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