PJB-2020-359
Genome-wide Analysis of DNA Methylation Patterning on Mutagenic Treatment of Alfalfa Using Whole-genome Bisulfite-Sequencing (BS-seq)
SHEN XIAOHUI
Abstract
To investigate the epigenetic effects of different mutagenesis treatments on alfalfa, dry alfalfa seeds were treated by EMS and 60Coγ-ray irradiation. Leaves of plants at seedling stage were obtained for genome-wide analysis of DNA methylation profiles by whole-genome bisulfite-sequencing (Bs-seq). The purpose of this study was to explore the changes of DNA methylation in alfalfa genome and the regulation mechanism of physiologically related gene expression. Results showed that CHH ratio is much higher than CG and CHG sequences at methylation sites from to the methylation ratio analyze, and CHH under EMS treatment was the highest proportion (64.37%); DNA methylation of alfalfa genome mainly occurs in CG sequence from the methylation level analyze; Subsequently, we identified 1719(CK VS. EMS) and 1315 (CK VS. 60CO-γ) DMR-related genes (DMGs). KEGG analyses revealed that some DMGs were involved in nitrogen metabolism, Pyrimidine metabolism and Amino sugar and nucleotide sugar metabolism. The number of genes (33) involved in chemical mutagenesis was higher than that involved in radiation mutagenesis (9). It indicates that the probability of differential methylation in chemical mutagenesis treatment is higher than that in radiation mutagenesis treatment. This research will provide an important basis for the molecular mechanism of the epigenetic in alfalfa induced by mutagenesis and for screening of alfalfa cold-resistant materials.
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