PJB-2020-502
Assessing the Genetic Diversity of Ginseng Populations in China by using ISSR Markers
Shi-jie WANG
Abstract
Inter-simple sequence repeat (ISSR) markers were used to assess the genetic diversity and population structure of seven ginseng populations in China. Ten of the 50 primers screened produced highly reproducible ISSR bands. Using these primers, 67 discernible DNA fragments were generated, 46 of which (68.65%) were polymorphic, indicating considerable genetic variation at the species level. In contrast, there were relatively low levels of polymorphism at the population level, with the percentage of polymorphic bands ranging from 47.12 to 53.12%. The mean gene diversity (He) estimated was 0.1112 within populations (ranging from 0.063 to 0.158) and 0.2196 at the species level. The values obtained for Shannon’s index (53.30%) indicated a high level of genetic differentiation among examined populations of ginseng in Northeast China.
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