Online Submission

Register | Forgot password?

Recent Issues

Uploaded: 01/06/2021 Uploaded: 02/04/2021
Uploaded: 09/03/2021 Uploaded: 09/03/2021

Latest News
Thursday, March 30, 2017

ISI Impact Factor 0.972


Paper Details


A comparative study of Euphorbia peplus, Euphorbia hirta and Euphorbia tirucalli based on DNA barcoding markers

Shawkat M. Ahmed and Ahmed Noureldeen


DNA regions (ITS, ITS2, matK, rbcL and trnH) have been examined for authentication and differentiation between E. peplus, E. hirta and E. tirucalli collected from Taif-KSA. The sequences of Euphorbia species were submitted in Genbank as new records within this research. ITS, ITS2 and rbcL provided good identification and clear resolution for the species, whereas, ITS2 and rbcL showed no or very little evolution. The highest sequence lengths were detected by matK and IT’S in E. hirta, E. tirucalli and then E. peplus. Our gathered results of Tajima relative evolutionary values revealed that ITS, matK and trnH showed rates of acceleration of evolution (P-values<0.05) within their sequences. The phylogenetic trees did not show any variability between the three Euphorbia species and the other retrieved GenBank accessions rejecting their endemism to flora of Saudi Arabia. The results support the hypothesis that E. peplus, E. hirta and E. tirucalli occur in the natural areas of Taif as naturalized species.  

To Cite this article: Ahmed, S.M. and A. Noureldeen. 2022. A comparative study of Euphorbia peplus, Euphorbia hirta and Euphorbia tirucalli based on DNA barcoding markers. Pak. J. Bot., 54(2): DOI:  

About Journal
Author's Guideline
Submit Online
In Press Articles
Status of Manuscript
Current Impact Factor 0.972
5 Years Impact Factor 0.85

PJB is indexed in following international databases: View all


Thomson Reuters ISI Web of Knowledge





International Scientific Indexing (ISI)

Cambridge Scientific Abstract

Chemical Abstract Services


ISC (Islamic World Science Citation Center)

Scientific Indexing Services


Index Copernicus (IC)



Google Scholar

Contact Us
Pakistan Journal of Botany, Botanical Garden, University of Karachi, Karachi, Pakistan | Facebook
Hit Counter: 3232444, Today's: 1428, Yesterday's: 1916, Country Wise Counter

Copyright © 2017-18 - All Rights Reserved -