PJB-2021-51
2,4-epibrassinolide relieves the damage of drought stress on fresh-cut lilies by increasing antioxidant enzyme activities
Wen Jinfen, Chi Bowen, Peng Chunxiu and Deng Minghua
Abstract
Fresh-cut flowers are often suffered from drought stress after they are harvested, which results in quality loss and vase life shorten during marketing of fresh-cut flowers. In this study, the lilies gathered from the greenhouse were treated with 10-7 M 2,4-epibrassinolide (EBR), and then subjected to drought treatment to assess the changes in wilting time, malondialdehyde (MDA) contents, relative conductivity, the total phenol (TP), antioxidant enzyme activities and these enzyme genes expression levels. Drought considerably accelerated the flowers wilting compared to the control. Moreover, extensive increase by drought in MDA content, and relative conductivity were also recorded. While the TP contents and the activities of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD); ascorbate peroxidase (APX) were firstly increased and then dropped in the later period. In which it seemed that SOD and POD played more important roles when the flowers were suffered drought stress. The expression of LbAPX, LbCu-ZnSOD, LbMn-SOD and LbCAT showed a rise and then decline, LbFe-SOD kept decreasing, while LbPOD maintained increasing. However, exogenous application of EBR remarkably delayed the flowers wilting time by 2 days and decreased the MDA content, relative conductivity compared to the drought group. These meant EBR treatment could maintain the membrane integrity of fresh-cut lilies. Furthermore, pre-treatment with EBR could significantly improve the TP contents and the four antioxidant enzymes activities and their genes expression levels. On the basis of these findings, it can be concluded exogenous application of EBR could effectively alleviate the damage of drought stress by reducing MDA contents and relative conductivity, improving TP content, antioxidant enzymes’ activities and their genes expressions, and finally extending the flowers’ post-harvest lives
To Cite this article:
Jinfen, W., C. Bowen, P. Chunxiu and D. Minghua. 2022. 2,4-epibrassinolide relieves the damage of drought stress on fresh-cut lilies by increasing antioxidant enzyme activities. Pak. J. Bot., 54(6): DOI: http://dx.doi.org/10.30848/PJB2022-6(1)
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