PJB-2021-86
Cloning and expression of floral organ identity genes in Paeonia ostii ‘Fengdan’
Shuang Zhou, Chao Ma, Shuangcheng Gao, Dianyun Hou, Peng Song, Zhenzhu Guo, Yi Zhang and Guoan Shi
Abstract
Tree peony is the most popular and important ornamental plant in China and has many different flower types. Although many studies about tree peony cultivation have been published, the regulatory mechanism of floral organ identity has not been explored. Paeonia ostii ‘Fengdan’ is a typical single-flower variety, and many important tree peony cultivars in China originated from homoploid hybridization between ‘Fengdan’ and other Paeonia species. Peony tea made from ‘Fengdan’ has already been introduced to the market, and the quality and price of peony tea are closely related to the flower type of ‘Fengdan’. This research cloned six floral organ identity genes in ‘Fengdan’, namely, PsAP1fd, PsAP2fd, PsAP3fd, PsPIfd, PsAGfd, and PsSEP1fd. The bioinformatics analysis was performed, and results showed that all these genes encoded MADS-box proteins, except PsAP2fd, and PsAP2fd encoded an AP2 protein. Five MADS-box proteins encoded by these genes contained two conserved motifs: MADS-MEF2 and K-box domain. PsSEP1fd was hydrophilic and stable, whereas the other five proteins were hydrophilic and unstable. The results of qRT-PCR displayed that PsAP1fd was mostly observed in the petals and sepals, and PsAP2fd was mostly found in the petals. PsAP3fd had strong expressions in the stamens and petals. The highest expression level of PsPIfd was observed in the petals, followed by that in the stamens. The highest expression level of PsAGfd was observed in the pistils, followed by that in the stamens. PsSEP1fd was mainly expressed in the pistils. These results showed that the ABCE model was effective in the flower type formation of ‘Fengdan’. Our work would help reveal the molecular mechanism underlying flower type formation in ‘Fengdan’ and promote quality control for peony tea products
To Cite this article:
Zhou, S., C. Ma, S. Gao, D. Hou, P. Song, Z. Guo, Y. Zhang and G. Shi. 2022. Cloning and expression of floral organ identity genes in Paeonia ostii ‘Fengdan’. Pak. J. Bot., 54(6): DOI: http://dx.doi.org/10.30848/PJB2022-6(27)
Download
