PJB-2021-183
Investigation of defense mechanism alterations induced by phytohormones, heat-shock and salt toxicity in green gram [Vigna radiata (L.) Wilczek]
Jabeen Farheen and Simeen Mansoor
Abstract
Salt toxicity cause a drastic reduction in green gram yield which plays a pivotal role in the human diet especially in vegetable-based society. With this respect, the destructive action from 50 to 350 mM NaCl and its recovery through phytohormones and heat-shock were evaluated on growth, biomolecules, and quality and quantity of DNA using advanced cost-effective and quick plant DNA extraction method. The salicylic acid (SA) 50 μM, indoleacetic acid (IAA) 100 μM, and gibberellic acid (GAᴣ) 100 μM were used as seed soaking (24 h) treatment before 40ºC heat-shock (1 h) then salt stress (144 h) applied on Vigna radiata (green gram) variety NM-92. The growth restraining impact of salt along with 40ºC was quite evident with enhancing NaCl concentrations. The utmost growth, and least malondialdehyde (8.76 μM gˉ¹ FW), proline (16.31 μM gˉ¹ FW), and hydrogen peroxide (7.01 μM gˉ¹ FW) were related to SA+30°C+NaCl 50 mM. While, ascorbate peroxidase, catalase, and superoxide dismutase maximum activities were associated with SA+30°C+NaCl 350 mM. The modified extraction method-2 yields high-quality DNA without liquid nitrogen, CTAB, Proteinase K, and prolong heating (65ºC). This method is ideal for the extraction of a large number of samples and storage for a year. DNA extraction results showed a 1.8 ratio at from all treatments that is suitable for RAPD-PCR reaction. Conclusively, 50 μM SA presoaking enhanced DNA stability under extreme salt stress that can be beneficial to grow green gram in the highly saline-affected zone
To Cite this article:
Farheen, J. and S. Mansoor. 2022. Investigation of defense mechanism alterations induced by phytohormones, heat-shock and salt toxicity in green gram [Vigna radiata (L.) Wilczek]. Pak. J. Bot., 54(6): DOI: http://dx.doi.org/10.30848/PJB2022-6(36)
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