PJB-2006-108
FACILE PLANT REGENERATION FROM TOMATO LEAVES INDUCED WITH SPECTINOMYCIN
MUHAMMAD SARWAR KHAN*, MUHAMMAD USMAN AND MUHAMMAD ILYAS LILLA
Abstract
Several studies have been conducted to find a combination of hormones conducive to improve regeneration from different explant tissues, particularly from leaves of tomato, and various responses were recorded to use in experiments. Major focus was to regenerate maximum number of shoots from tomato leaves Cv. Moneymaker, Packet, Nagina and Aroma. Depending on the cultivar used, shoot regeneration varied (Moneymaker = Packet = Nagina = Aroma) on RMOT medium (MS salts supplemented with 1mg/L Zeatin and 1mg/L IAA). In addition, several other media were used to compare regeneration efficiencies including RMOP medium containing MS salts supplemented with 0.1mg/L NAA
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