PJB-2021-340
Using genetic and CRISPR/Cas9 methods to achieve quadruple mutant in trichome trimeric complex for future study in fiber initiation mechanism
Trinh Ngoc Ai, Nghi Khac Nhu and Anh Phu Nam Bui
Abstract
Despite many similarities between the molecular machinery for initiation in cotton fiber and Arabidopsis trichome, there are significant distinctions in how fiber and trichome pattern. While trichome initiation are clearly specified, there is a randomness in fiber initiation in seed coat. While characterizing the cotton fiber genes, the corresponding Arabidopsis gene is replaced by the cotton homolog, and all the complemented lines showed a defined trichome pattern similar to wild type Arabidopsis. Owing to the lack of appropriate tools, the fundamental dissimilarities in the patterning process remain unresolved. To completely understand the cotton fiber initiation mechanism and the difference in pattern formation between cotton fiber and Arabidopsis trichome, we aimed to create a cotton trimeric complex MYB2-DEL65/DEL61-TTG3 in Arabidopsis to study the fiber initiation mechanism. To achieve this ultimate objective, the first step is to generate Arabidopsis quadruple mutant (gl1gl3egl3ttg1). In this paper we successfully applied genetic and CRISPR/Cas9 approaches to obtain the quadruple mutant. Further, we also introduced the cotton MYB2-A gene into the quadruple mutant. The material generated by this paper will subsequently be used to decipher the molecular basis for cotton fiber initiation
To Cite this article:
Ai, T.N., N.K. Nhu and A.P.N. Bui. 2024. Using genetic and CRISPR/Cas9 methods to achieve quadruple mutant in trichome trimeric complex for future study in fiber initiation mechanism. Pak. J. Bot., 56(2): DOI: http://dx.doi.org/10.30848/PJB2024-2(7)
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