Cloning, characterization, and expression analysis of two MAPKKK genes in Chrysanthemum
Ren Liping, Yin Dandan, Wan Wenyang, Fang Tingting, Su Xiaohui and Cao Xiaohan
MAPKKK (mitogen-activated protein kinase kinase kinase) genes are involved in plant growth and stress responses.The expression profiles of MAPKKK genes in response to various stimuli, including high temperature, drought, and salt, as well as different hormones, were investigated in this study using a Chrysanthemum morifolium variety called 'Jinba' (such as abscisic acid, methyl jasmonate, salicylic acid and ethylene). According to our previous transcriptome data, we identified and cloned two MAPKKK genes with complete open reading frames. These two MAPKKK genes were studied in bioinformatics and expression models in response to various stimuli. Both of these genes belonged to the MEKK subfamily, according to cluster analysis. They were most abundantly expressed in the leaves, according to expression analysis, and their levels of expression were subjected to treatments. Our findings suggest that CmMAPKKK genes may be involved in multiple stress responses and hormone responses, which will facilitate our future researches on their functions.
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