PJB-2021-413
The comparative alpha-amylase inhibitory and antioxidant potentials of different extracts of Capparis decidua fruit of Pakistani origin
Bushra Asghar, Syed Saeed Ul Hassan, Muhammad Abbas, Muhammad Abuzar Ghaffari, Hammad Ahmed and Muhammad Khalil-Ur-Rehman
Abstract
Use of plant materials, extracts and bioactive compounds termed traditional practice. This type of exercise provided less expensive remedies and better health care. Therapeutic characteristics of natural herbs are usually joined to the secondary metabolites. Traditional medicinal plants have a significant role to manage diabetes mellitus. Consequently, our study is mainly focused on the evaluation of phytochemicals, antioxidant and anti-diabetic properties of Capparis decidua fruit as well as comparative study of its different extracts, such as; n-hexane (NCD), chloroform (CCD) and methanolic (MCD). The phytochemical investigation of C. decidua confirmed the presence of carbohydrates, alkaloids, proteins, glycosides tannins and fixed oils. In addition, total phenolic and flavonoids contents were also assessed and found chief quantity in NCD extract. DPPH protocol was employed to evaluate the anti-oxidant activity with all extracts. The percentage-inhibitions 60.58%, 56.88% and 47.62% exhibited with NCD, CCD and MCD respectively. Values of IC50 were observed, 977.4μg/mL (NCD), 1214μg/mL (CCD) and 3011μg/mL (MCD). Anti-diabetic activity (In-vitro) was calculated through α-amylase inhibition protocols. The percentage suppressions with MCD, CCD and NCD were observed 80.56, 83.18 and 86.11% respectively. The IC50 values with all said extracts 605, 456 and 167 μg/mL were analyzed. The comparative antioxidant and anti-diabetic aptitudes were also examined and concluded NCD found significant potentials
To Cite this article:
Asghar, B., S. Saeed-Ul-Hassan, M. Abbas, M.A. Ghaffari, H. Ahmed and M. Khalil-Ur-Rehman. 2022. The comparative alpha-amylase inhibitory and antioxidant potentials of different extracts of Capparis decidua fruit of Pakistani origin. Pak. J. Bot., 54(6): DOI: http://dx.doi.org/10.30848/PJB2022-6(42)
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