Effect of mutation induced by gamma-irradiation in ornamental plant lilium (Lilium longiflorum cv. Tresor)
Hanifeh Seyed Hajizadeh, Seyed Najmedin Mortazavi, Farzaneh Tohidi, Hasibe Yildiz, Murat Helvaci, Turgut Alas and Volkan Okatan
Genetic diversity and its utilization in plant breeding is the main objective. Induction of Mutation has known as the major pathway for the induction of genetic diversity in flowers. In order to study the effect of gamma rays on the mutagenicity of lily cut flower a project was carried out in a completely randomized design with tree replications. Lilium bulbs were irradiated with six different levels of gamma rays (control, 10, 20, 30, 40 and 50 Gy). Then the number of leaves, leaf area, fresh and dry weight, stem height, leaf relative water content, ion leakage, chlorophyll a and b and total chlorophyll were measured. Irradiation results showed a significant difference in leaf number, ion leakage and chlorophyll a, b and total. In this GR50 most mutations occur in the sample and the damage will be minimal (growth reduction by 50%). Due to high coefficient of determination (R2) obtained in dry weight; this factor is used to determine the GR50 compared with other characters is more suitable. The optimal dose of radiation at the plant was computed between 22.5-27.5 Gy. The consequence of treatment on leaf relative water content was not significant. In general High-gamma irradiation doses had harmful effects on growth of Lilium.
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