PJB-2021-435
Cloning of CmMYB1 from Cucumis melo L. and its functional roles under salt stress
Huijun Zhang
Abstract
MYB transcription factor plays an important role in plant adaptation to abiotic stress, especially in response to salt stress.CmMYB1 homologous gene in muskmelon (Cucumis melo L.) was obtained by homologous cloning, introducing the CmAMYB1 gene by Agrobacterium-mediated transformation. The results of subcellular localization showed that the CmMYB1 protein was expressed in cytoplasm and nucleus. CmMYB1 gene was introduced into wild-type A. thaliana by agrobacterium-mediated transformation, and the growth status of T2 transgenic A. thaliana and wild-type A. thaliana was observed. The results showed that compared with the wild-type, the transgenic A. thaliana phenotype did not change significantly.After treatment with 200mM NaCl solution for 1h and 3h, the CmMYB1 gene expression increased significantly and began to decline after 6h of salt stress, indicating that it functions in the early response to salt stress.These results indicate that CmMYB1 gene is involved in salt stress. In addition, we also observed the phenotypic changes of CmMYB1 overexpressing Arabidopsis thaliana (CmMYB1-OX) and wild-type Arabidopsis thaliana under salt stress for 14 days, which provided a theoretical basis for further study of the biological function of cmmyb1.
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