PJB-2021-492
Biofilm inhibition and antibacterial potential of bio-functional components from selected medicinal plants against sinusitis isolates.
Muhammad Asif Rafiq
Abstract
Biofilm is an intricate bacterial consortium extremely impervious to antibiotics. Due to the development of resistance against the commonly used synthetic antibiotics researcher are looking for new remedies from natural medicinal plants for the treatment of bacterial infections. This research work was planned to study the antimicrobial activity and the biofilm inhibition potential of selected medicinal plants against sinusitis isolates. All four plant extracts Cuminum cyminum (Cumin seed), Zingiber officinale (zinger roots), Cinnamomum verum (cinnamon bark), and Elettaria cardamom (Green cardamom fruit) exhibited significant antibacterial activity through agar well diffusion assay. Combined water extract of plants showed a synergistic antibacterial effect in terms of the better zone of inhibition ranges from 13.6 to 17.5 mm. MIC determined through the microtiter dilution method ranges from 2.5 to 0.625 mg / mL for combined aqueous extract. Biofilm inhibition was performed by crystal violet assay. A mixture of water extract showed significant anti-biofilm activity ranging from 45 to 64 % which suggested that combined water extract might act as a potential compound against sinusitis isolates. The phase-contrast microscopic study also indicated the biofilm inhibition potential. The present work concluded that combined water extract of four medicinal plants showed good antibacterial and anti-biofilm potential and should be further evaluated for compounds that actually gave synergistic effects.
To Cite this article:
Download
