PJB-2021-511
EFFECT OF A NOVEL COCOS NUCIFERA (COCONUT) OIL BASED EMULSION ON PCR EFFICIENCY
Hafsa Ahmed Khan
Abstract
Emulsion Polymerase Chain Reaction (ePCR) is a well-known technique allowing for the creation of in-vitro compartments. This compartmentalization helps in reducing PCR biasness by making it possible for more and all kinds of DNA templates to find their way into a polymerase chain reaction. It also works to reduce intermediate product formation. The water-in-oil emulsion technique gives rise to droplets of enough stability and strength that each one of them ends up acting as a complete PCR itself. In this study, we explored the potentials of using an easy mix of emulsion for ePCR. We developed a novel formula employing drug store bought Cocos Nucifera oil, more commonly called Coconut oil, as an alternative to Mineral Oil in emulsions for ePCR. Our studies concluded that ePCR does result in performing the polymerase chain reaction with the Coconut Oil based emulsions. The novel emulsion developed was not only workable, but also produced efficient results for the amplified DNA. The percentage increase trends for the total amplified DNA product obtained at the end of different ePCR runs were observed quantitatively using Image J analysis and densitometry calculations.
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