PJB-2021-634
Molecular identification of Colletotrichum capsici isolates and development of mathematical model for chilli anthracnose dynamics in semi-arid climate of the Punjab, Pakistan
Abid Riaz, Yasin, M. Sheeraz Ahmad, Abdul Sattar and Nasir Mehmood
Abstract
Anthracnose is one of the major constraints to chilli production globally, especially in tropical and subtropical regions. Exact identification of Colletotrichum isolates from Pakistan and anthracnose dependence on weather parameters in semi-arid condition necessitated conducting work on molecular identification of the isolates and epidemiological aspects of the disease. DNA of five representative samples from five districts of the Punjab was extracted, purified and amplified by PCR. The purified PCR products were sequenced and submitted to NCBI GenBank database to obtained the accession numbers viz. MG799567, MG799563, MG799564, MG799565 and MG799566 were obtained for ITS region while MK088521, MK088522, MK088523, MK088524 and MK088525 for Beta tubulin gene. After confirmation of the isolates, sequences were uploaded on NCBI for BLAST to determine the similarity of the sequences of subjected isolates to previously reported sequences. Molecular characteristics of the pathogen resulted in confirmation of Colletotrichum capsici as causal agent of the chilli anthracnose in Punjab, Pakistan. Relationship of weather parameters with disease for two years in the form of mathematical model suggested that all the three factors (mean minimum and maximum temperatures and relative humidity) play significant role in disease dynamics. For most of the time period, weather conditions have been conducive for anthracnose development suggesting that researchers should pay more attention to develop and introduce anthracnose resistant chilli varieties for this zone.
To Cite this article:
Riaz, A., Yasin, M.S. Ahmad, A. Sattar and N. Mehmood. 2023. Molecular identification of Colletotrichum capsici isolates and development of mathematical model for chilli anthracnose dynamics in semi-arid climate of the Punjab, Pakistan. Pak. J. Bot., 55(4): DOI: http://dx.doi.org/10.30848/PJB2023-4(31)
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