PJB-2021-680
ESTABLISHMENT OF REGENERATION SYSTEM FOR TISSUE CULTURE OF Euphorbia milii
Lou Xiyan
Abstract
The stem segments, leaves and bud tip of Euphorbia milii were used as explants to explore the effects of different hormone combinations on the callus induction, differentiation and rooting, and effects of different culture substrates on the survival rate of transplanting. The results showed that the induction of stem segments was the easiest, and the callus induction was best in the hormone ratio of MS+1.5 mg/L 6-BA+0.1 mg/L NAA，and the induction rate was 85.17% . The effect of adventitious buds induction was best in the hormone ratio of MS+1.5mg/L 6-BA+0.1mg/L NAA and the average proliferation coefficient reached 4.67. The effect of rooting of adventitious bud was best in the ratio of 1/2MS+0.2 mg/L IBA hormone, and the average rooting coefficient reached 5.72. For transplanting of plantlets, the cultivation effect was best in the culture substrate of nutrient soil: vermiculite: sand = 1:1:1, and the survival rate reached 96%.
To Cite this article:
Download
