PJB-2022-38
Exploring the role of WRKY transcription factor in the growth and development of Bletilla striata based on bioinformatics analysis
Qingqing Li
Abstract
Abstract: Key message: To explore the genetic mechanism of regulating growth and chemical biosynthesis is vital important for further studies and applications in medicinal herbs. By applied bioinformatic programs, we screened 29 WRKY genes from former formed database and then identified their functions based on the sequences properties. Backgrounds: WRKY type transcription factors (TFs) play crucial roles in the growth and development of plants. However, a comprehensive analysis of the WRKY family members in a valuable Chinese herbal orchid, Bletilla striata, or in other orchids, is limited. Methods: In this study, WRKY gene family was screened out from the transcriptome data of Bletilla striata by bioinformatics method. A series of bioinformatic tools were applied to explore the properties of sequences and characterize their functions. The EST-SSR markers were detected and further confirmed by PAGE assays. Results and conclusions: The 29 WRKY TFs that were identified from the B. striata genome and named BsWRKY1 to BsWRKY29 were divided into three clades: group â… (involving 8 WRKY sequences), group â…¡ (18) and group â…¢ (3), in which group â…¡ was further divided into 5 subgroups: â…¡-a (involving 1 WRKY sequences), â…¡-b (5), and â…¡-c (3), â…¡-d (7), â…¡-e (3). EST-SSR marker mining test showed that 10 markers could be stably amplified with obvious polymorphisms among 4 landraces. Our data suggest that BsWRKY genes may work together to regulate plant growth and development. In different subcellular locations, BsWRKY genes not only played its own functions, but also coordinated the regulation of the whole life activities. Taken together, these results provided a theoretical basis for further studies on the gene functions and regulatory mechanisms of what in B. striata.
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