PJB-2022-57
Identification and characterization of serine proteases from Cuminum cyminum (Apiaceae)
Uzma Zaman, Atiya Abbasi and Saima Mahmood Malhi
Abstract
Cuminum cyminum L. of the family Apiaceae, commonly known as cumin or safaid zeera, has been popularly utilized as common spice around the world. Phytochemical analysis and pharmacological studies proved the presence of several biological active compounds with health beneficial effects. The present work aimed to identify the proteases in the aqueous extract of Cuminum cyminum. Total proteins were extracted and fractionated by size exclusion chromatography. Protein fractions were divided in two groups CSP I and CSP II on the basis of protein pattern and the presence of protease activities by SDS-PAGE and zymography, respectively. Additionally, both group showed optimum activity at pH range 5-6 and at 35oC using casein as substrate. Significant inhibition of activity by phenylmethylsulfonyl fluoride, indicated that enzymes of both groups belonged to the family of plant serine proteases. These cumin proteases can be a significant addition in plant proteases family which is in continuous growth due to their wide range of application in industries like food, leather, detergent and pharmaceutical manufacturing, offering substitute to the harmful chemicals
To Cite this article:
Zaman, U., A. Abbasi and S.M. Malhi. 2024. Identification and characterization of serine proteases from Cuminum cyminum (Apiaceae). Pak. J. Bot., 56(1): DOI: http://dx.doi.org/10.30848/PJB2024-1(2)
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