PJB-2022-93
In Vitro Anti-Bacterial Activity and Isolation of Active Secondary Metabolites from Oligomeris Linifolia (Vahl Ex Hornm) J.F. Macbr from Upper Dir, KP, Pakistan
Ayesha Ayub
Abstract
In the present study, we investigated Oligomeris linifolia for antibacterial activity by using the well diffusion method against four selected bacterial strains named: Escherichia coli, Methicillin-resistant Staphylococcus aureus, Salmonella typhi, and Pseudomonas aeruginosa. The leaf extract of the plant showed significantly high antibacterial activity against all tested bacterial strains as compared to positive control in which Vancomycin is used. The leaves’ extract showed a 24 mm zone of inhibition against S.T. 23 mm E. coli, 19 mm MRSA, and 19 mm against P.A. The bio autographic analysis of leaf methanolic extract also revealed significant antibacterial activity against tested bacterial strains. Thin Layer Chromatography (TLC) results showed 5 bands in the order of decreasing values. These five bands were evident in the TLC plate visualized under the visible light. It is discovered that a new compound flavanol which showed 15 mm (Rf 13) zone of inhibition against S.T, 13 mm (Rf 15) MRSA, 13.66 mm (Rf 12) and 13 mm (Rf 15) against P.A. E. Therefore, it is concluded from the current study that leaf extract of this plant has potential to develop antimicrobial drugs.
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