PJB-2022-105
Analysis of Leaf Trait and Differentially Expressed Genes of Leaf Development in Eucommia ulmoides Oliver after Ultraviolet-B (UV-B) Radiation
Dewen Li
Abstract
Eucommia ulmoides Oliver (EUO) has a great medicinal value in China. The using leaf model of Eucommia planting (ULMEP) is a promising planting model to resolve the shortage of EUO resources. We focused on leaf responses and differentially expressed genes (DEGs) for transcriptome profiling after ultraviolet-B (UV-B) radiation in the east-north China. The results showed that leaf traits were significantly affected after ultraviolet-B (UV-B) radiation, and the leaf area, leaf thickness and total leaf fresh/dry weight significantly increased (p<0.05). Anatomical results showed that cells from the leaf epidermal and palisade tissue were larger than those of control. Two transcriptome databases were integrated and assembled, comparative analysis of both transcriptome databases revealed 1,826 up-regulated and 1,872 down-regulated genes. Among them, the up-regulated unigenes were mainly involved in Signal transduction mechanisms [T], and Cell wall/membrane biogenesis [M] by the KOG database, and up-regulated genes were analyzed in plant hormone signal transduction and phenylpropanoid biosynthesis metabolic pathways by KEGG pathway. Furthermore, 10 candidate unigenes were randomly selected to examine gene expression using RT-qPCR, the two libraries exhibited differential expression and were identical to those obtained by sequencing (Fig. 11). Thus, the data in this study are helpful to clarify the reasons why leaf growth after UV-B radiation. This study provides a valuable reference for the ULMEP in the east-north China.
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