PJB-2022-142
IDENTIFICATION OF WHEAT TaP5CS GENE AND FUNCTIONAL ANALYSIS UNDER DROUGHT STRESS CONDITIONS
Chunhua Wang
Abstract
Wheat (Triticum. aestivum L.) is one of the major cereal crops cultivated largely worldwide. Drought is an important factor affecting wheat yields and quality. The screening of drought resistance genes and analyzing drought resistance mechanisms can provide candidate genes and a reference basis for wheat drought resistance molecular breeding. Δ 1-pyrroline-5-carboxylic acid synthetase (P5CS) is a key enzyme of proline metabolism and proline is a key regulator of osmotic stability in plants subjected to drought stress. In this study, we generated a cDNA copy of the P5CS gene from the drought-tolerant wheat variety Qingmai 6 and introduced the gene into Arabinoses thaliana and tested whether the drought tolerance of these transgenic plants was enhanced compared with their wildtype counterparts. TaP5CS was predicted to be a cytoplasmic protein in wheat and this was verified in transgenic plants. Homology analysis of the TaP5CS protein sequence indicated that it belonged to the ProB super-family and was most closely related to its ortholog from Aegilops aegerita. Moreover, quantitative real-time PCR (qRT-PCR) analysis showed that the expression of TaP5CS was strongly induced in leaves under 4℃, ABA, PEG and NaCl stress conditions. We found that over-expression of TaP5CS in A. thaliana increased the drought resistance by increasing the proline content, SOD and CAT activity. This study provides useful information for the use of the wheat TaP5CS gene in enhancing drought resistance and provides a theoretical basis for the regulation of wheat resistance to abiotic stressors.
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