PJB-2022-160
PRODUCTION OPTIMIZATION OF AMYLASE FROM INDIGENOUS BACTERIA USING BANANA PEELS. AN APPROACH TO REDUCE AND UTILIZE AGRO-INDUSTRIAL WASTE
MADIHA MUHAMMAD FAZIL
Abstract
Alpha amylases are starch hydrolyzing enzymes that possess high industrial demand. Various strategies have been adopted to enhance cost-effective enzyme production of which utilization of agro-industrial waste is very promising. In this study two amylase producers; Bacillus megaterium and Exiguobacterium auranticum were isolated and identified by 16sRNA sequencing, their growth conditions were optimized via submerged fermentation using banana peels as carbon source. Bacillus megaterium found to be mesophilic alkaline (37℃, pH of 7 to 9) strain giving 475 U/ml while Exiguobacterium auranticum found to be acidic thermophilic (50°C, pH 5) that gave 400 U/ml. Production of amylase by acidic thermophilic E. auranticum is reported here for the first time. The crude enzyme also showed better chocolate and curry stain removal capacity when combined with commercial detergent showing their potential to be useful in the detergent industry.
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