PJB-2022-1545
An efficient micropropagation of Reseda lutea: a rare plant of Saudi Arabia
Fahad Al-Qurainy, Saleh Alansi, Salim Khan, Mohammad Nadeem, Aref Al-Shameri, Mohamed Tarroum, Abdel-Rhman Z. Gaafar and Nora Al-Farraj
Abstract
The goal of this work was to look at the propagation of Reseda lutea L. by organogenesis in tissue culture. Explants from In vitro grown seedlings were taken from the axillary bud. After seven days of culture on MS medium supplemented with 1.0 mg/L BAP, the adventitious buds developed. After three weeks of culturing on MS medium supplemented with 1.5 mg/L BAP, the maximum multiplication of shoots (16.12 shoots/explant) was discovered, with an average (7.37 cm) shoots/explant. These shoots were sub-cultured on MS media with varying concentrations of NAA and IBA for root initiation. The MS medium combined with IBA produced the greatest percentage of root development (92%) and the greatest number of roots (7.37 roots/plant). In MS media supplemented with 0.5 NAA, the longest roots (3.08 cm) were found. After 17 days in a glasshouse, the plantlets were acclimatized in pots containing Peat moss and pearlite, 98 percent of the plantlets were acclimatized. To get a plant in a pot, the complete procedure took about 75 days. The technique proposed could aid in the preservation of the plant both In vivo and In vitro
To Cite this article:
Al-Qurainy, F., S. Alansi, S. Khan, M. Nadeem, A. Al-Shameri, M. Tarroum, A.R.Z. Gaafar and N. Al-Farraj. 2022. An efficient micropropagation of Reseda lutea: a rare plant of Saudi Arabia. Pak. J. Bot., 54(6): DOI: http://dx.doi.org/10.30848/PJB2022-6(21)
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