PJB-2022-188
Optimization of desk-type scanning electronic microscopy in observing the leaves of Camellia weiningensis
Li Long
Abstract
Camellia weiningensis has high ornamental and economic values. In this study, we aimed to find the optimal parameters of scanning electronic microscopy (SEM) for the observation of C. weiningensis leaves by comparing the imaging effects under different working conditions. In the presence of gold coating instrument, a backscattered electron (BSE) probe, 10-KV voltage and moderate vacuum should be selected. The order of the influence of the working parameters on imaging effect was probe> voltage>vacuum degree. In the absence of gold coating instrument, a BSE probe, 10-KV voltage and low vacuum should be selected. The uncoated samples presented with the most severe damage in the high vacuum mode; the leaves became loose and soft and the surface curled with cracks. In the best working parameter mode, the time duration of gold coating was 45 s, which ensured observation effect and reduced energy waste.
To Cite this article:
Download
