PJB-2022-335
GENOME-WIDE ANALYSES OF CORE REGULATORY MODULE SHATTERING CASCADEGENES IN CANOLA (BRASSICA NAPUS L.)
Muhammad Yasin
Abstract
Premature seed shattering in canola is resulting in huge losses in yield up to 50% in adverse climatic conditions. In model plant Arabidopsis thaliana, which belong to same family Brassicaceae, 8 genes have been found to be included in shattering cascade. Among these, 3 genes including SHATTERPROOF1, SHATTERPROOF2 and FRUITFUL (SHP1/2-FUL) belonging to MADS-box family implicated in fruit dehiscence zone and valve margin constitute a core regulatory module. But in Brassica, the exact number of genes involved in shattering remained obscure.
BnSHP1-N, BnSHP2-N and BnFUL-N were grouped into their respective clades based on phylogenetic reconstruction of core regulatory modules (SHP1, SHP2 and FUL) and from other species' homologs. The 8 shattering cascade genes did not show any conservation indicating their involvement in shattering the exons and introns in gene structure analysis. Comparative genome synteny through separate pathways. Increased in number of homologs/paralogs in Brassica was due to occurrences of genome duplication or triplication event during evolution. Exonization and intronization could be responsible for variable number and of size of analysis of SHP1, SHP2 and FUL revealed correlation and evolutionary insights into genes regions relationships in the entire Brassicaceae. Our results exhibit basic information on cloning, phylogenetic re-construction, genomics loci and identification of transcription factor-binding sites (TFBSs) of core regulatory module genes that might be helpful for developing shattering resistant genome edited plants to prevent yield losses in canola in future.
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