PJB-2022-350
EVALUATION AND COMPARISON OF THE ANTIOXIDANT AND RADICAL SCAVENGING PROPERTIES OF MEDICINAL PLANTS BY USING THE DPPH ASSAY IN-VITRO
Syed Anis Ali Jafri
Abstract
Introduction: Free radicals have been found to cause a number of problems and damage in the human body especially cardiovascular disorders and cancer. These radiation-based treatments may have some damaging effects on other normal body cells. So there is a need to explore some natural means which may contain antioxidants, which can trap the ROS produced within the living body. Methods: In this research, various medicinal plant samples were observed for free radical hunting or scavenging activity of their methanolic, ethanolic and aqueous extracts by using the DPPH (1, 1- diphenyl, 2-picryl-hydrazyl) assay in-vitro by taking the absorbance reading at 517nm using a spectrophotometer, because of flavonoids and other polyphenol contents, the anti-oxidant activity was possessed by these traditionally used medicinal plants from Himalayan regions of Pakistan. Results: The results showed that all samples in ethanolic extracts showed a higher value of radical scavenging potential. The highest radical scavenging activity was observed in the Euphrasiae stricta (IC50= 38.972 μg/mL), Euphorbia platyphyllos L. (IC50= 40.817 μg/mL), and Epimedium brevicomum Maxim (IC50= 46.265 μg/mL), medicinal plants for both of their ethanolic and methanolic extracts as compared to the Ascorbic Acid scavenging activity (IC50=37.337 μg/ml). Conclusion: These Plants can be efficiently applied as an important antioxidant source for the treatment and inhibition of widely spread oxidative stress-related degenerative diseases like cancer, cardiovascular & inflammatory disorders, atherosclerosis, dementia, diabetes, asthma, and eyes related to degenerative diseases, etc.
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