PJB-2022-494
Green and efficient extraction of 10-deacetylbaccatin and cephalomanine by deep eutectic solvents from Taxus chinensis
Jafar Khan, Shakir Ullah, Khizar Hayat, Uzma Salam, Shahid Ali and Yu-Jie Fu
Abstract
The green extraction of plant bioactive compounds is intensively needed at present. Many efforts have been practiced so far to seek greenish and environmentally benign substitutes to conventional solvents to avoid or reduce the hazardous effects of the extraction media. Microwave irradiation extraction in combination with deep eutectic solvents is used in the current study to extract 10-DAB III and cephalomanine from Taxus chinensis. Eight deep eutectic solvents were evaluated with betaine as a hydrogen bond acceptor and various hydrogen bond donors such as polyalcohol, organic acids, and sugars. As a controlled solvent, ethanol 80% (v/v) was used. Our findings revealed that Be-lactic acid had the maximum extraction yield of 10-DAB III (0.85 mg/g dry weight) and cephalomanine (0.80 mg/g dry weight), in contrast with conventional solvents (0.67 mg/g dry weight) and (0.30 mg/g dry weight), respectively. The extraction yield was increased significantly when the Be:LA molar ratio (mol:mol) was increased to 1:3 (0.94 mg 10-DAB III and 1.10 mg cephalomanine/g dw, respectively). Besides this, different extraction methods were also evaluated for comparative assessment, which showed the significant superiority of the DES-MAE (microwave-assisted extraction) method in extracting the target compounds compared to the DES-UAE (ultrasound-assisted extraction) and DES-HRE (heat reflux extraction) method. It is concluded that deep eutectic solvents can be an easy, reliable, safe, cheaper, and efficient extraction medium in T. chinensis plants compared to conventional solvents
To Cite this article:
Khan, J., S. Ullah, K. Hayat, U. Salam, S. Ali and Y.J. Fu. 2024. Green and efficient extraction of 10-deacetylbaccatin and cephalomanine by deep eutectic solvents from Taxus chinensis. Pak. J. Bot., 56(2): DOI: http://dx.doi.org/10.30848/PJB2024-2(13)
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