Paper Details

PJB-2023-19

Comparative analysis of main active constituents of Mume Fructus and from different drying methods by ultra-high performance liquid chromatography with triple quadrupole tandem mass spectrometry 

Nannan Li
Abstract


A rapid, sensitive and convenient analytical method of ultra-performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry (UPLC-QTRAP-MS /MS ) was proposed for the simultaneous determination of characteristic components covering many structure types including organic acids, flavonoids and amino acids in Mume Fructus (MF). The proposed method was first reported and validated by assessing the matrix effects, linearity, limit of detections, limit of quantifications, precision, repeatability, stability and recovery of target components.The developed UPLC-QTRAP-MS/MS was successfully applied to simultaneously determine. All target compounds in 12 batches of MF from different drying methods.  In order to comprehensively evaluate the quality of MF, principal component analysis (PCA) method were conducted on the data of the contents of 47 active components. The proposed approach could be readily utilized as a comprehensive approach for determining the consistency of the quality and therapeutic efficacy of MF. 

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