PJB-2023-137
Physiological response and differential gene expression analysis of Hemiptelea davidii (Ulmaceae) seedlings under PEG stress
Chengjun Yang, Jiayi Shen, Jingwen Shang and Tiantian Yang
Abstract
Hemiptelea davidii is a drought-tolerant, sand-fixing, wind-preventing, and versatile plant that is highly adaptable to growing in harsh terrain such as barren hills and sandy areas. In this study, PEG-6000 solution was used to simulate drought in young Hemiptelea davidii seedlings to investigate the changes of chlorophyll, osmoregulation substances, malondialdehyde (MDA) and antioxidant enzyme activities in the leaves at four PEG concentrations (0, 10%, 20% and 30%). Hemiptelea davidii was stressed with high concentration of 30% PEG for 0h, 6h and 12h, and its leaves were collected for transcriptome sequencing to investigate the molecular mechanism of Hemiptelea davidii under drought stress. The experiment shows that Hemiptelea davidii can resist a certain extent and duration of drought. However, under high concentration and long-term drought, its defense system will be destroyed and disordered, and its drought resistance will decrease. A total of 54,598 Unigene were assembled by transcriptome sequencing analysis of Hemiptelea davidii leaves, and 56.4% were found to be annotated with major databases. The transcripts showed that 620 and 3803 differentially expressed genes (DEGs) were detected in w1_w2 and w1_w3, respectively. GO, COG and KEGG analyses showed that PEG stress induced the expression of genes related to photosynthesis and metabolic pathways. Transcription factor analysis revealed a total of 63 transcription factor families, including C2H2, bHLH, AP2/ERF-ERF, etc. These transcription factor genes were involved in plant hormone regulation, growth and development, and might play an essential role in Hemiptelea davidii coping with PEG stress. The study of the drought-tolerant mechanism of Hemiptelea davidii provide a basis for popularizing the planting of Hemiptelea davidii and excavating the stress-resistant genes of Hemiptelea davidii.
To Cite this article:
Yang, C., J. Shen, J. Shang and T. Yang. 2024. Physiological response and differential gene expression analysis of Hemiptelea davidii (Ulmaceae) seedlings under PEG stress. Pak. J. Bot., 56(2): DOI: http://dx.doi.org/10.30848/PJB2024-2(42)
Download
