PJB-2023-192
Application of ITS2 secondary structure in species identification and classification of Rehmannia
Xiaoru Cheng, Haoyuan Li, Jiuchang Su, Peilei Chen, Wanshen Wang, Yousef Sultan and Hongying Duan
Abstract
The internal transcribed spacer 2 (ITS2) sequence is known for its remarkable ability to distinguish between plant species, making it a valuable tool for phylogenetic analysis and species classification. In this investigation, we elucidated the secondary structure of ITS2 in Rehmannia, revealing a characteristic arrangement of four helices. It's noteworthy that the length and shape of each helix vary, contributing to significant interspecific differences. Furthermore, certain variants of R. glutinosa displayed notable disparities in their ITS2 secondary structures. Additionally, when comparing ITS2 sequences and secondary structures, we observed distinct differences between Rehmannia and its closely related genus, Triaenophora. This divergence highlighted the potential of ITS2, not only for genetic information but also for its structural characteristics, in enhancing species discrimination. Consequently, our findings underscore the substantial promise of ITS2 in the realms of species classification and identification
To Cite this article:
Cheng, X., H. Li, J. Su, P. Chen, W. Wang, Y. Sultan and H. Duan. 2024. Application of ITS2 secondary structure in species identification and classification of Rehmannia. Pak. J. Bot., 56(6): DOI: http://dx.doi.org/10.30848/PJB2024-6(27)
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